{"product_id":"senataxin-antibody-setx-bha17136294","title":"Senataxin Antibody \/ SETX","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eSenataxin Antibody \/ SETX is a anti-SETX Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e SETX\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eSenataxin antibody detects DNA\/RNA helicase Senataxin, encoded by the SETX gene located on chromosome 9q34.13. Senataxin is a large nuclear helicase that unwinds DNA\/RNA hybrids known as R-loops, thereby maintaining genomic stability during transcription and replication. It plays crucial roles in transcription termination, RNA processing, and DNA damage repair, linking transcriptional control with genome maintenance. Senataxin is expressed widely, with elevated levels in neurons, germ cells, and proliferative tissues where transcription-coupled DNA repair is highly active.\u003cbr\u003e\u003cbr\u003eStructurally, Senataxin is a 2677-amino-acid helicase belonging to the superfamily 1 (SF1) of DNA\/RNA helicases. It contains a C-terminal helicase domain with conserved Walker A and B motifs for ATP binding and hydrolysis, an N-terminal region that mediates protein-protein interactions, and nuclear localization sequences. The helicase activity of Senataxin resolves R-loop structures formed during transcription, preventing collisions between transcription and replication machinery. Co-localization studies show Senataxin enriched in nuclear foci associated with sites of active transcription and DNA repair.\u003cbr\u003e\u003cbr\u003eFunctionally, Senataxin maintains genome integrity by resolving R-loops and facilitating proper termination of RNA polymerase II transcription. It contributes to DNA double-strand break repair via homologous recombination and regulates transcription-coupled nucleotide excision repair. Senataxin also modulates RNA maturation by interacting with RNA processing factors such as XRN2 and the exosome complex. In neurons, it supports axonal maintenance and stress granule dynamics, while in germ cells, it regulates meiotic recombination and spermatogenesis.\u003cbr\u003e\u003cbr\u003eMutations in the SETX gene are linked to several neurodegenerative and neuromuscular disorders, including ataxia with oculomotor apraxia type 2 (AOA2) and juvenile amyotrophic lateral sclerosis type 4 (ALS4). These conditions are characterized by progressive neurodegeneration and impaired DNA repair capacity. Senataxin deficiency leads to accumulation of R-loops, transcriptional stress, and genomic instability. Pathway associations include RNA processing, DNA damage response, and transcription regulation. During development, Senataxin expression supports neuronal differentiation and genome maintenance in post-mitotic cells.\u003cbr\u003e\u003cbr\u003eThe Senataxin antibody from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=SETX - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=SETX - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=SETX - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/SETX - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=SETX+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047325131117,"sku":"FY13393","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_99b21639-b11a-4e35-8abb-08e8c7f681d9.jpg?v=1782237104","url":"https:\/\/www.ebiohippo.com\/products\/senataxin-antibody-setx-bha17136294","provider":"BioHippo","version":"1.0","type":"link"}