{"product_id":"serpina12-antibody-serpin-a12-vaspin-bha17135667","title":"SERPINA12 Antibody \/ Serpin A12 \/ Vaspin","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eSERPINA12 Antibody \/ Serpin A12 \/ Vaspin is a anti-SERPINA12 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e SERPINA12\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eSERPINA12 antibody detects Vaspin (serpin A12), a secreted serine protease inhibitor that modulates insulin sensitivity, inflammation, and metabolic balance. Encoded by the SERPINA12 gene on chromosome 14q32.13, this protein belongs to the serpin (serine protease inhibitor) superfamily, known for their roles in controlling proteolytic cascades. Vaspin is predominantly expressed in visceral adipose tissue, liver, and pancreas, and circulates as an adipokine that improves insulin sensitivity and exerts anti-inflammatory effects. Its expression is influenced by obesity, metabolic stress, and insulin signaling, suggesting a compensatory role in metabolic homeostasis.\u003cbr\u003e\u003cbr\u003eSERPINA12 inhibits proteases that degrade insulin-sensitizing factors and inflammatory mediators, contributing to glucose regulation and vascular protection. Experimental models demonstrate that Vaspin administration improves glucose tolerance and reduces insulin resistance in obese and diabetic subjects. Mechanistically, it modulates serine protease activity in adipose tissue and the liver, attenuating inflammatory cytokine production and endothelial dysfunction. It may also interact with GRP78 on cell surfaces to activate downstream signaling that promotes insulin sensitivity and cellular protection.\u003cbr\u003e\u003cbr\u003eThe SERPINA12 antibody is widely used in endocrinology, metabolism, and cardiovascular research to study the regulation and function of Vaspin. Western blot analysis typically identifies a 45 kilodalton band corresponding to the unglycosylated form of the protein, while immunohistochemistry shows cytoplasmic and extracellular staining in adipose and hepatic tissues. This antibody supports investigation of the molecular mechanisms underlying insulin resistance, obesity, and metabolic inflammation.\u003cbr\u003e\u003cbr\u003eBeyond metabolic regulation, SERPINA12 is associated with cardiovascular protection through inhibition of vascular inflammation and endothelial apoptosis. It may also have roles in tissue repair and cancer progression.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=SERPINA12 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=SERPINA12 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=SERPINA12 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/SERPINA12 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=SERPINA12+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047299113325,"sku":"FY12764","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_98888370-9dc4-4103-951e-efb80d1ff63a.jpg?v=1782237064","url":"https:\/\/www.ebiohippo.com\/products\/serpina12-antibody-serpin-a12-vaspin-bha17135667","provider":"BioHippo","version":"1.0","type":"link"}