{"product_id":"serpinb12-antibody-serpin-family-b-member-12-bha17135587","title":"SERPINB12 Antibody \/ Serpin family B member 12","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eSERPINB12 Antibody \/ Serpin family B member 12 is a anti-SERPINB12 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e SERPINB12\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eSERPINB12 antibody targets Serpin family B member 12, a cytoplasmic serine protease inhibitor belonging to the clade B (ovalbumin-like) subfamily of serpins. These intracellular serpins modulate proteolytic activity to maintain cellular homeostasis, control inflammation, and prevent premature cell death caused by uncontrolled protease action. SERPINB12, encoded by the SERPINB12 gene on chromosome 18q21.33, is expressed in epithelial tissues such as the liver, pancreas, kidney, and gastrointestinal tract. The protein functions primarily as an inhibitor of trypsin-like serine proteases, including granzyme B and trypsin, protecting cells from protease-mediated damage during stress or immune response.\u003cbr\u003e\u003cbr\u003eStructurally, SERPINB12 consists of a conserved serpin fold comprising three beta-sheets and nine alpha-helices surrounding a reactive center loop (RCL). Upon cleavage by a target protease, SERPINB12 undergoes an irreversible conformational change that traps the enzyme in a covalent complex, neutralizing its activity. This suicide-inhibition mechanism is characteristic of the serpin family and critical for regulating proteolytic cascades within cells. In the pancreas and gastrointestinal tract, SERPINB12 prevents inappropriate activation of digestive enzymes that could cause tissue injury. In immune contexts, it protects epithelial cells against cytotoxic proteases released by immune effectors.\u003cbr\u003e\u003cbr\u003eBeyond its role in protease inhibition, SERPINB12 has emerging significance in cancer biology and tissue homeostasis. Overexpression of SERPINB12 has been reported in hepatocellular carcinoma, pancreatic ductal adenocarcinoma, and colorectal cancer, where it may contribute to cell survival under inflammatory or proteolytic stress. Conversely, loss of SERPINB12 function has been associated with enhanced susceptibility to apoptosis and tissue injury. The SERPINB12 antibody is widely used in studies examining protease regulation, epithelial defense, and tumor biology. Immunohistochemistry with this antibody reveals cytoplasmic localization consistent with its role as an intracellular serpin. In biochemical assays, western blotting identifies a 42 kilodalton band corresponding to SERPINB12, confirming its specificity.\u003cbr\u003e\u003cbr\u003eFunctional studies have shown that SERPINB12 may cooperate with other serpins, such as SERPINB9 and SERPINB13, to establish a network of intracellular protease control in secretory epithelia. This redundancy ensures protection against multiple classes of serine proteases during inflammation or infection. In model systems, SERPINB12 expression is upregulated by cytokines and growth factors, suggesting regulation through stress-responsive pathways. The SERPINB12 antibody thus provides a valuable tool for mapping these protective responses and for characterizing epithelial resilience to proteolytic challenge. Its expression profile also makes it a potential biomarker for epithelial tumor progression and inflammation-associated pathology.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=SERPINB12 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=SERPINB12 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=SERPINB12 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/SERPINB12 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=SERPINB12+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047296033133,"sku":"FY12684","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_0f94a63b-4107-4f09-ba04-08d606651d6b.jpg?v=1782237030","url":"https:\/\/www.ebiohippo.com\/products\/serpinb12-antibody-serpin-family-b-member-12-bha17135587","provider":"BioHippo","version":"1.0","type":"link"}