{"product_id":"sesn2-antibody-sestrin-2-bha17136113","title":"SESN2 Antibody \/ Sestrin 2","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eSESN2 Antibody \/ Sestrin 2 is a anti-SESN2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human. Reported localization: Cytoplasm.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e SESN2\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eSESN2 antibody detects Sestrin 2, a stress-inducible metabolic regulator that protects cells from oxidative damage and maintains energy balance through AMPK and mTOR signaling. The UniProt recommended name is Sestrin 2 (SESN2). This cytoplasmic protein belongs to the sestrin family of stress-responsive factors that restore redox homeostasis and suppress excessive growth signaling under nutrient limitation or oxidative stress.\u003cbr\u003e\u003cbr\u003eFunctionally, SESN2 antibody identifies a 480-amino-acid protein induced by p53, FOXO, and ATF4 transcription factors in response to stress stimuli such as DNA damage, hypoxia, and reactive oxygen species. SESN2 activates AMP-activated protein kinase (AMPK) and inhibits mammalian target of rapamycin complex 1 (mTORC1) by interacting with the GATOR2 complex, thereby reducing anabolic activity and promoting autophagy. It also regenerates overoxidized peroxiredoxins, restoring cellular antioxidant capacity and maintaining metabolic equilibrium.\u003cbr\u003e\u003cbr\u003eThe SESN2 gene is located on chromosome 1p35.3 and is expressed in metabolically active tissues such as liver, skeletal muscle, and heart. Its expression levels increase during fasting, exercise, or oxidative stress, making SESN2 a crucial component of the cellular adaptive stress response.\u003cbr\u003e\u003cbr\u003ePathologically, dysregulation of SESN2 contributes to aging, metabolic disorders, and cancer. Reduced SESN2 activity promotes oxidative stress, mitochondrial dysfunction, and insulin resistance, while its overexpression has protective effects against neurodegeneration and tissue injury. Research using SESN2 antibody supports studies in autophagy, metabolism, and stress signaling pathways.\u003cbr\u003e\u003cbr\u003eSESN2 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect oxidative stress regulators. \u003cbr\u003e\u003cbr\u003eStructurally, Sestrin 2 contains a conserved catalytic domain with oxidoreductase-like motifs, though it lacks enzymatic activity. Instead, its structure supports binding to GATOR complexes and peroxiredoxins. This antibody facilitates detailed investigation of SESN2's role in energy sensing, antioxidant defense, and lifespan regulation.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=SESN2 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=SESN2 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=SESN2 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/SESN2 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=SESN2+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047318577517,"sku":"FY13211","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_2e5f95a2-6563-4dd2-8731-52d7e597a3cc.jpg?v=1782237076","url":"https:\/\/www.ebiohippo.com\/products\/sesn2-antibody-sestrin-2-bha17136113","provider":"BioHippo","version":"1.0","type":"link"}