{"product_id":"sesn3-antibody-sestrin-3-bha17135281","title":"SESN3 Antibody \/ Sestrin 3","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eSESN3 Antibody \/ Sestrin 3 is a anti-SESN3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e SESN3\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC\/IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eThe SESN3 antibody targets Sestrin-3, a stress-responsive protein encoded by the SESN3 gene that acts as a key regulator of metabolism, oxidative stress, and autophagy. Sestrin-3 belongs to the sestrin family of highly conserved proteins that modulate AMPK and mTORC1 signaling in response to cellular stress and nutrient availability. By integrating metabolic and redox cues, Sestrin-3 protects cells from oxidative damage and supports energy balance. The SESN3 antibody provides a high-specificity reagent for studying these protective pathways and their dysregulation in metabolic and age-related diseases.\u003cbr\u003e\u003cbr\u003eSestrin-3 functions as both an antioxidant and a signaling adaptor. It promotes activation of AMP-activated protein kinase (AMPK) and inhibits mechanistic target of rapamycin complex 1 (mTORC1), thereby promoting autophagy and metabolic homeostasis under nutrient stress. The SESN3 antibody enables detection of this adaptive protein across a range of tissues, including liver, muscle, adipose, and brain, where it contributes to maintaining glucose and lipid metabolism. Through these pathways, Sestrin-3 serves as a critical mediator of cellular recovery following oxidative or genotoxic stress.\u003cbr\u003e\u003cbr\u003eIn metabolic research, Sestrin-3 has gained prominence as a regulator of insulin sensitivity and lipid catabolism. Elevated SESN3 expression improves glucose tolerance and suppresses lipogenesis through AMPK activation. Conversely, its loss results in impaired energy metabolism and increased susceptibility to metabolic syndrome. The SESN3 antibody supports studies of these mechanisms by quantifying protein levels in metabolic tissues and cell lines subjected to nutrient deprivation or stress stimuli.\u003cbr\u003e\u003cbr\u003eSestrin-3 also contributes to antioxidant defense by reducing reactive oxygen species (ROS) accumulation and supporting regeneration of peroxiredoxins. These functions are critical in preventing oxidative stress�induced damage in aging, cardiovascular disease, and neurodegeneration. The SESN3 antibody allows detailed analysis of these processes and helps map signaling interactions with FOXO and p53 transcription factors that drive stress-response gene expression. Dysregulation of Sestrin-3 has been implicated in tumor progression, where its expression may modulate cell survival under metabolic stress conditions common in cancer microenvironments.\u003cbr\u003e\u003cbr\u003eThe SESN3 antibody performs effectively in western blotting, immunohistochemistry, and immunofluorescence. It provides clear cytoplasmic staining consistent with the localization of this stress-inducible regulator.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=SESN3 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=SESN3 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=SESN3 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/SESN3 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=SESN3+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047288725869,"sku":"FY12378","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_973fb749-536a-4669-94f7-ebe9a8d076f5.jpg?v=1782237055","url":"https:\/\/www.ebiohippo.com\/products\/sesn3-antibody-sestrin-3-bha17135281","provider":"BioHippo","version":"1.0","type":"link"}