{"product_id":"setbp1-antibody-set-binding-protein-1-bha17135443","title":"SETBP1 Antibody \/ SET binding protein 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eSETBP1 Antibody \/ SET binding protein 1 is a anti-SETBP1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e SETBP1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC\/IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eSETBP1 antibody detects SET binding protein 1, a nuclear protein that interacts with the SET oncoprotein to regulate chromatin structure, gene expression, and cellular proliferation. SETBP1 modulates transcriptional programs controlling stem cell maintenance and oncogenic transformation. The SETBP1 antibody is used in studies of leukemia, developmental syndromes, and transcriptional regulation.\u003cbr\u003e\u003cbr\u003eSETBP1 is encoded by the SETBP1 gene on human chromosome 18q12.3. The protein is approximately 170 kilodaltons and contains multiple AT-hook motifs for DNA binding, nuclear localization signals, and a SET-binding domain that mediates interaction with the SET protein, a known inhibitor of protein phosphatase 2A (PP2A). Through this interaction, SETBP1 indirectly modulates PP2A activity, influencing cell cycle progression and epigenetic control.\u003cbr\u003e\u003cbr\u003eThe SETBP1 antibody detects a 145-150 kilodalton band by western blot and shows nuclear localization under immunofluorescence. SETBP1 acts as a transcriptional regulator that binds GC-rich promoter regions to enhance expression of target genes, including HOXA cluster members associated with hematopoietic differentiation and leukemogenesis. Mutations that stabilize SETBP1 lead to aberrant accumulation, transcriptional reprogramming, and malignant transformation in myelodysplastic syndromes and acute myeloid leukemia.\u003cbr\u003e\u003cbr\u003eIn addition to cancer, germline mutations in SETBP1 cause Schinzel-Giedion syndrome, a severe developmental disorder characterized by craniofacial abnormalities and neurological impairment. These mutations increase SETBP1 stability, disrupt normal transcriptional repression, and impair neuronal differentiation. SETBP1 also interacts with chromatin-modifying complexes to coordinate histone acetylation and methylation status.\u003cbr\u003e\u003cbr\u003eAs a molecular bridge between SET and chromatin, SETBP1 integrates signaling pathways that regulate proliferation, apoptosis, and gene expression.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=SETBP1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=SETBP1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=SETBP1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/SETBP1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=SETBP1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047292363117,"sku":"FY12540","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_15e108b7-7855-4095-a2f1-12e4c1813f15.jpg?v=1782237026","url":"https:\/\/www.ebiohippo.com\/products\/setbp1-antibody-set-binding-protein-1-bha17135443","provider":"BioHippo","version":"1.0","type":"link"}