{"product_id":"setd5-antibody-set-domain-containing-protein-5-bha17135809","title":"SETD5 Antibody \/ SET domain-containing protein 5","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eSETD5 Antibody \/ SET domain-containing protein 5 is a anti-SETD5 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e SETD5\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eSETD5 antibody detects SET domain-containing protein 5, a chromatin-associated enzyme implicated in epigenetic regulation, transcriptional control, and neurodevelopment. Encoded by the SETD5 gene on chromosome 3p25.3, this protein contains a conserved SET (Su(var)3-9, Enhancer-of-zeste, Trithorax) domain characteristic of lysine methyltransferases. Although its precise enzymatic targets remain under investigation, SETD5 is thought to influence chromatin structure and gene accessibility by interacting with histone-modifying and transcriptional complexes.\u003cbr\u003e\u003cbr\u003eStructurally, SETD5 is a large nuclear protein of approximately 1,442 amino acids and 170 kilodaltons containing an N-terminal SET domain and coiled-coil regions that mediate interactions with epigenetic cofactors such as HDAC3 and PAF1C. SETD5 localizes primarily to euchromatin, where it contributes to transcriptional repression and developmental gene regulation. It is broadly expressed, with highest levels in brain and embryonic tissues, supporting its essential role in differentiation and neurogenesis.\u003cbr\u003e\u003cbr\u003eThe SETD5 antibody is widely used in epigenetics, neuroscience, and developmental biology research to study chromatin remodeling, gene silencing, and neurodevelopmental disorders. Western blot analysis detects a 170 kilodalton band corresponding to SETD5, while immunofluorescence demonstrates nuclear localization with punctate chromatin-associated staining. This antibody provides a reliable reagent for assessing epigenetic enzyme expression and chromatin regulatory function.\u003cbr\u003e\u003cbr\u003eLoss-of-function mutations in SETD5 are associated with autosomal dominant intellectual disability and developmental delay syndromes. Functional studies show that SETD5 regulates genes involved in cell cycle control, neural differentiation, and synapse formation through coordination of histone deacetylation and RNA polymerase II elongation. It acts as an integrator of epigenetic and transcriptional machinery, maintaining proper expression of developmental regulators. Dysregulated SETD5 expression has been linked to tumor progression, particularly in cancers with altered chromatin modification landscapes. The SETD5 antibody provides an essential tool for exploring the interplay between chromatin dynamics, transcriptional control, and development.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=SETD5 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=SETD5 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=SETD5 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/SETD5 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=SETD5+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047305568621,"sku":"FY12907","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_13f45121-5c0a-4f13-9824-f90d2d81ee5d.jpg?v=1782237055","url":"https:\/\/www.ebiohippo.com\/products\/setd5-antibody-set-domain-containing-protein-5-bha17135809","provider":"BioHippo","version":"1.0","type":"link"}