{"product_id":"sfmbt2-antibody-scm-like-with-four-mbt-domains-protein-2-bha17135908","title":"SFMBT2 Antibody \/ Scm-like with four MBT domains protein 2","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eSFMBT2 Antibody \/ Scm-like with four MBT domains protein 2 is a anti-SFMBT2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e SFMBT2\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC\/IF, IP, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eSFMBT2 antibody detects Scm-like with four MBT domains protein 2, a chromatin-associated transcriptional repressor involved in epigenetic gene silencing. The UniProt recommended name is Scm-like with four MBT domains protein 2 (SFMBT2). This protein belongs to the polycomb group (PcG)-related MBT family, which regulates gene expression through chromatin compaction and histone modification recognition.\u003cbr\u003e\u003cbr\u003eFunctionally, SFMBT2 antibody identifies a 1220-amino-acid nuclear protein that binds to methylated histones, particularly histone H3 dimethylated on lysine 9 (H3K9me2) and lysine 27 (H3K27me2). SFMBT2 functions as a transcriptional corepressor by stabilizing repressive chromatin domains, thereby restricting transcriptional activation of developmental and cell cycle genes. It is a structural homolog of Drosophila Scm (Sex comb on midleg), which participates in Polycomb repressive complex formation.\u003cbr\u003e\u003cbr\u003eThe SFMBT2 gene is located on chromosome 8q13.3 and encodes a protein widely expressed in embryonic and adult tissues, with enrichment in stem cells and reproductive organs. SFMBT2 plays a key role in early embryogenesis and trophoblast differentiation by maintaining chromatin architecture and silencing specific developmental genes. In trophoblast stem cells, SFMBT2 contributes to maintenance of stemness and regulation of imprinted gene expression.\u003cbr\u003e\u003cbr\u003eEpigenetically, SFMBT2 interacts with histone deacetylases (HDACs) and other repressive complexes to enforce gene silencing. It binds methylated histones through its MBT (malignant brain tumor) domains, which function as methyl-lysine recognition modules. Loss or depletion of SFMBT2 leads to de-repression of silenced loci, chromatin decondensation, and transcriptional misregulation. In cancer, altered SFMBT2 expression has been observed in hepatocellular carcinoma and prostate cancer, implicating it in tumor growth and chromatin remodeling.\u003cbr\u003e\u003cbr\u003eSFMBT2 antibody is widely used in epigenetics, chromatin biology, and transcriptional regulation studies. It is suitable for immunoblotting, chromatin immunoprecipitation (ChIP), and immunofluorescence to investigate SFMBT2 localization and histone interactions. This antibody aids in exploring PcG-mediated gene repression and chromatin dynamics in development and disease. It also helps characterize the role of SFMBT2 in maintaining heterochromatin and transcriptional stability.\u003cbr\u003e\u003cbr\u003eStructurally, SFMBT2 contains four MBT domains arranged in tandem that form a histone-binding scaffold, along with N-terminal coiled-coil motifs that mediate protein-protein interactions. Post-translational regulation includes phosphorylation and ubiquitination, which may influence chromatin association.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=SFMBT2 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=SFMBT2 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=SFMBT2 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/SFMBT2 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=SFMBT2+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047308943725,"sku":"FY13006","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_b50e58bf-2a4f-4a7c-8bdd-5025612cf1e6.jpg?v=1782237076","url":"https:\/\/www.ebiohippo.com\/products\/sfmbt2-antibody-scm-like-with-four-mbt-domains-protein-2-bha17135908","provider":"BioHippo","version":"1.0","type":"link"}