{"product_id":"sfxn4-antibody-sideroflexin-4-bha17135881","title":"SFXN4 Antibody \/ Sideroflexin 4","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eSFXN4 Antibody \/ Sideroflexin 4 is a anti-SFXN4 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm (Mitochondria).\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e SFXN4\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, IF, ICC\/IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eSFXN4 antibody detects Sideroflexin-4, a mitochondrial inner membrane protein involved in iron-sulfur cluster biogenesis and mitochondrial respiration. The UniProt recommended name is Sideroflexin-4 (SFXN4), a member of the sideroflexin family of transporters that regulate iron metabolism and mitochondrial energy production. SFXN4 plays a crucial role in maintaining mitochondrial homeostasis by supporting the synthesis and incorporation of iron-sulfur cofactors into respiratory chain complexes.\u003cbr\u003e\u003cbr\u003eFunctionally, SFXN4 antibody identifies a 317-amino-acid transmembrane protein that contributes to the maturation of mitochondrial iron-sulfur (Fe-S) proteins. SFXN4 facilitates iron import into the mitochondrial matrix and interacts with key components of the Fe-S assembly machinery, including ISCU and NFS1. Through this interaction, it ensures the proper incorporation of Fe-S clusters into enzymes critical for electron transport, oxidative phosphorylation, and metabolic regulation. Disruption of SFXN4 impairs mitochondrial respiration, leading to decreased ATP production and increased oxidative stress.\u003cbr\u003e\u003cbr\u003eThe SFXN4 gene is located on chromosome 10q26.3 and encodes a protein with multiple transmembrane domains localized to the inner mitochondrial membrane. It belongs to the sideroflexin family, which includes five human homologs (SFXN1-SFXN5) with distinct but overlapping roles in amino acid and iron transport. Among these, SFXN4 has a specialized role in mitochondrial iron utilization and heme biosynthesis. Loss-of-function mutations in SFXN4 cause mitochondrial complex I and III deficiencies, resulting in mitochondrial myopathy, anemia, and developmental delay.\u003cbr\u003e\u003cbr\u003eIn normal physiology, SFXN4 maintains iron homeostasis and supports mitochondrial protein synthesis. It is essential for cell survival under metabolic stress conditions requiring efficient oxidative phosphorylation. Dysfunction of SFXN4 affects the activity of Fe-S cluster-dependent enzymes such as aconitase and succinate dehydrogenase, contributing to defects in the tricarboxylic acid (TCA) cycle and respiratory chain function.\u003cbr\u003e\u003cbr\u003eSFXN4 antibody is widely used in mitochondrial biology, metabolism, and bioenergetics research. It is valuable for western blotting, immunofluorescence, and mitochondrial fractionation studies to analyze SFXN4 expression and localization. In disease research, this antibody helps investigate mitochondrial dysfunctions linked to anemia, neurodegeneration, and metabolic syndromes. Reduced SFXN4 expression is associated with impaired mitochondrial translation and decreased oxidative capacity, while overexpression may alter cellular redox balance.\u003cbr\u003e\u003cbr\u003eStructurally, SFXN4 contains multiple hydrophobic helices forming a membrane-spanning topology that mediates metabolite and ion transport. Its function depends on coordination with mitochondrial carrier proteins and iron chaperones.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=SFXN4 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=SFXN4 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=SFXN4 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/SFXN4 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=SFXN4+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047307567469,"sku":"FY12979","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_0633f3d7-8cb7-4f78-83ab-2fcb05f16480.jpg?v=1782237067","url":"https:\/\/www.ebiohippo.com\/products\/sfxn4-antibody-sideroflexin-4-bha17135881","provider":"BioHippo","version":"1.0","type":"link"}