{"product_id":"shank1-antibody-sh3-and-multiple-ankyrin-repeat-domains-protein-1-bha17135367","title":"SHANK1 Antibody \/ SH3 and multiple ankyrin repeat domains protein 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eSHANK1 Antibody \/ SH3 and multiple ankyrin repeat domains protein 1 is a anti-SHANK1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e SHANK1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC\/IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eSHANK1 antibody recognizes SH3 and multiple ankyrin repeat domains protein 1, a large scaffolding protein of the postsynaptic density (PSD) essential for excitatory synapse organization and signaling. SHANK1 belongs to the SHANK family, which also includes SHANK2 and SHANK3, each coordinating complex protein-protein interactions that anchor neurotransmitter receptors and cytoskeletal elements at synapses. The SHANK1 antibody is a crucial reagent for studying synaptic plasticity, neurodevelopmental disorders, and molecular mechanisms underlying learning and memory.\u003cbr\u003e\u003cbr\u003eSHANK1 is encoded by the SHANK1 gene located on human chromosome 19q13.33. The protein exceeds 200 kDa and contains several structural domains: multiple ankyrin repeats, an SH3 domain, a PDZ domain, a proline-rich region that interacts with cortactin and Homer, and a sterile alpha motif (SAM) that mediates self-association. These domains enable SHANK1 to function as a scaffold linking glutamate receptors, adhesion molecules, and signaling enzymes into large PSD assemblies. In neurons, SHANK1 stabilizes AMPA and NMDA receptor complexes and regulates dendritic spine morphology.\u003cbr\u003e\u003cbr\u003eThe SHANK1 antibody is commonly used to label postsynaptic structures, showing punctate localization in dendritic spines consistent with PSD distribution. Western blot analysis typically reveals a prominent band between 240-260 kDa. Functional studies demonstrate that SHANK1 promotes synapse maturation and coordinates actin cytoskeleton dynamics, thereby influencing neuronal connectivity and excitatory signaling. Mutations or deletions of SHANK family genes are associated with autism spectrum disorder and intellectual disability, underscoring the functional importance of these scaffolding proteins in brain development.\u003cbr\u003e\u003cbr\u003eBeyond the nervous system, SHANK1 expression has been observed in testis and other tissues where it may regulate cytoskeletal organization. Interaction partners include PSD-95, GKAP, Homer, and cortactin, forming a structural network essential for synaptic signal transduction. Loss of SHANK1 disrupts synaptic transmission and reduces spine density, while overexpression alters synaptic scaling and excitatory balance.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=SHANK1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=SHANK1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=SHANK1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/SHANK1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=SHANK1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047289807213,"sku":"FY12464","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_2185ba28-53da-4ff6-9506-8da55b455681.jpg?v=1782237005","url":"https:\/\/www.ebiohippo.com\/products\/shank1-antibody-sh3-and-multiple-ankyrin-repeat-domains-protein-1-bha17135367","provider":"BioHippo","version":"1.0","type":"link"}