{"product_id":"shank2-antibody-sh3-and-multiple-ankyrin-repeat-domains-protein-2-bha17135857","title":"SHANK2 Antibody \/ SH3 and multiple ankyrin repeat domains protein 2","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eSHANK2 Antibody \/ SH3 and multiple ankyrin repeat domains protein 2 is a anti-SHANK2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e SHANK2\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eSHANK2 antibody detects SH3 and multiple ankyrin repeat domains protein 2, a scaffolding protein of the postsynaptic density (PSD) that organizes synaptic signaling complexes. The UniProt recommended name is SH3 and multiple ankyrin repeat domains protein 2 (SHANK2). This large multidomain protein serves as a structural hub connecting neurotransmitter receptors, ion channels, and cytoskeletal elements at excitatory synapses.\u003cbr\u003e\u003cbr\u003eFunctionally, SHANK2 antibody recognizes a synaptic scaffolding protein of approximately 180-200 kDa that coordinates synaptic architecture and signaling. SHANK2 interacts with PSD-95, Homer, and cortactin, linking surface glutamate receptors to the actin cytoskeleton. These interactions stabilize dendritic spine morphology and facilitate efficient synaptic transmission. SHANK2's PDZ, SAM, and ankyrin repeat domains enable it to integrate diverse protein-protein interactions, forming large macromolecular complexes essential for signal transduction.\u003cbr\u003e\u003cbr\u003eThe SHANK2 gene is located on chromosome 11q13.3 and encodes multiple isoforms through alternative splicing. SHANK2 is particularly abundant in the cerebral cortex, hippocampus, and cerebellum, where it regulates synaptic plasticity and neuronal connectivity. Genetic studies associate SHANK2 mutations and deletions with autism spectrum disorder (ASD), intellectual disability, and schizophrenia, linking its dysfunction to altered excitatory signaling and neural circuit development.\u003cbr\u003e\u003cbr\u003eSHANK2 antibody is a powerful tool for neuroscience research, enabling investigation of postsynaptic organization, receptor clustering, and synaptic remodeling. It is widely applied in western blotting, immunofluorescence, and co-immunoprecipitation to study excitatory synapses and protein interactions within the PSD. In neuronal cultures, SHANK2 colocalizes with synaptic markers such as PSD-95 and Synapsin, marking mature excitatory synapses. Loss of SHANK2 expression disrupts dendritic spine density and reduces AMPA and NMDA receptor-mediated currents, impairing synaptic strength.\u003cbr\u003e\u003cbr\u003eStructurally, SHANK2's N-terminal ankyrin repeats mediate cytoskeletal binding, while its C-terminal SAM domain enables oligomerization, forming mesh-like scaffolds beneath the postsynaptic membrane. Post-translational modifications, including phosphorylation and palmitoylation, regulate its localization and synaptic activity. SHANK2 also interacts with neuroligins and mGluR5, connecting receptor signaling to structural reorganization. Dysregulation of SHANK2 leads to impaired synaptic signaling and behavioral abnormalities in animal models.\u003cbr\u003e\u003cbr\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=SHANK2 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=SHANK2 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=SHANK2 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/SHANK2 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=SHANK2+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047307075949,"sku":"FY12955","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_7ab52d34-4813-4892-aab9-ee71cec2d790.jpg?v=1782237060","url":"https:\/\/www.ebiohippo.com\/products\/shank2-antibody-sh3-and-multiple-ankyrin-repeat-domains-protein-2-bha17135857","provider":"BioHippo","version":"1.0","type":"link"}