{"product_id":"sharpin-antibody-shank-associated-rh-domain-interacting-protein-bha17135864","title":"SHARPIN Antibody \/ SHANK-associated RH domain-interacting protein","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eSHARPIN Antibody \/ SHANK-associated RH domain-interacting protein is a anti-SHARPIN Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e SHARPIN\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eSHARPIN antibody detects SHANK-associated RH domain-interacting protein, a multifunctional adaptor protein involved in inflammation, apoptosis, and cytoskeletal signaling. The UniProt recommended name is SHANK-associated RH domain-interacting protein (SHARPIN). This cytoplasmic protein is a key component of the linear ubiquitin chain assembly complex (LUBAC), which modulates NF-kappaB activation and immune response signaling.\u003cbr\u003e\u003cbr\u003eFunctionally, SHARPIN antibody recognizes a 387-amino-acid protein that acts as a scaffolding subunit linking the ubiquitin ligase HOIP (RNF31) and HOIL-1L (RBCK1) to form the active LUBAC complex. This complex catalyzes linear (M1-linked) polyubiquitination of signaling proteins, stabilizing TNF receptor-associated complexes and promoting NF-kappaB activation. Through this pathway, SHARPIN regulates innate and adaptive immune responses, cell survival, and inflammatory gene transcription. It also negatively regulates apoptotic signaling by inhibiting caspase activation downstream of TNF receptors.\u003cbr\u003e\u003cbr\u003eThe SHARPIN gene is located on chromosome 8q24.3 and encodes a widely expressed cytoplasmic protein enriched in immune cells, skin, and neural tissues. Beyond immune regulation, SHARPIN interacts with integrins and actin-associated proteins to control cell adhesion and motility. It binds to the SHANK family of postsynaptic scaffolds, linking cytoskeletal dynamics to synaptic stability and plasticity. SHARPIN thus functions as both an immune modulator and structural adaptor in distinct cellular contexts.\u003cbr\u003e\u003cbr\u003eDeficiency or mutation of SHARPIN disrupts LUBAC assembly, leading to impaired NF-kappaB signaling and increased apoptosis. Mouse models lacking SHARPIN exhibit severe chronic inflammation and skin lesions, known as the chronic proliferative dermatitis (cpdm) phenotype. In humans, SHARPIN dysregulation has been implicated in inflammatory diseases, cancer, and neurodegeneration. Elevated SHARPIN expression correlates with tumor progression in prostate and breast cancers, where it enhances PI3K\/AKT signaling and resistance to apoptosis.\u003cbr\u003e\u003cbr\u003eSHARPIN antibody is widely used for research into inflammatory signaling, ubiquitination, and immune regulation. Applications include immunoblotting, immunohistochemistry, and co-immunoprecipitation to analyze LUBAC complex formation and function. Its detection provides insight into TNF receptor signaling, cell death control, and integrin-mediated adhesion. In neurobiology, SHARPIN interacts with SHANK scaffolds and PSD-95 at excitatory synapses, influencing postsynaptic organization and neuronal communication.\u003cbr\u003e\u003cbr\u003eStructurally, SHARPIN contains an N-terminal PH domain-like motif, a central ubiquitin-like (UBL) domain, and a C-terminal Npl4 zinc finger (NZF) domain responsible for binding linear ubiquitin chains. Post-translational regulation includes phosphorylation and ubiquitination, controlling its participation in signaling networks.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=SHARPIN - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=SHARPIN - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=SHARPIN - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/SHARPIN - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=SHARPIN+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047307600237,"sku":"FY12962","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_6e013819-5290-4965-94c7-f57edeef6558.jpg?v=1782237069","url":"https:\/\/www.ebiohippo.com\/products\/sharpin-antibody-shank-associated-rh-domain-interacting-protein-bha17135864","provider":"BioHippo","version":"1.0","type":"link"}