{"product_id":"shb-antibody-sh2-domain-containing-adapter-protein-b-bha17135282","title":"SHB Antibody \/ SH2 domain-containing adapter protein B","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eSHB Antibody \/ SH2 domain-containing adapter protein B is a anti-SHB Rabbit antibody Recombinant Rabbit Monoclonal clone 31S61 supplied in Liquid format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e SHB\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 31S61, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eSHB antibody detects SH2 domain containing adapter protein B, an adaptor protein that participates in signal transduction downstream of receptor and non receptor tyrosine kinases. SHB is involved in transmitting signals that regulate cell proliferation, differentiation, apoptosis, and cytoskeletal rearrangements. Through its SH2 domain, SHB interacts with phosphorylated tyrosine residues on activated receptors and kinases, thereby linking upstream cues to downstream signaling pathways.\u003cbr\u003e\u003cbr\u003eSHB antibody is widely used in studies of angiogenesis, immune regulation, and cancer. SHB is known to associate with vascular endothelial growth factor receptor signaling and contributes to endothelial cell migration, survival, and sprouting. In immune cells, SHB modulates T cell receptor signaling and influences activation, proliferation, and cytokine production. Altered expression of SHB has been associated with tumor development and progression, underscoring its importance as both a research target and potential biomarker.\u003cbr\u003e\u003cbr\u003eThe antibody is suitable for western blotting, immunohistochemistry, immunofluorescence, and flow cytometry. In western blot assays, SHB antibody detects protein bands of expected size, confirming its expression under different cellular conditions. Immunohistochemistry provides tissue distribution patterns, often highlighting expression in vascular structures and immune infiltrates. Immunofluorescence allows colocalization with kinases and receptors, demonstrating its adaptor role within signaling complexes. These applications make SHB antibody a versatile reagent for cell biology and disease research.\u003cbr\u003e\u003cbr\u003eSHB has been implicated in diabetes related vascular complications, where it affects insulin signaling and endothelial function. By employing SHB antibody, researchers can explore how adaptor proteins influence metabolic and vascular health. In cancer biology, SHB links growth factor receptor signaling to pathways that drive tumor angiogenesis and invasion. Detecting SHB expression can therefore provide insight into tumor progression and therapy resistance.\u003cbr\u003e\u003cbr\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=SHB - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=SHB - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=SHB - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/SHB - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=SHB+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047286923629,"sku":"FY12379","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_628e1023-08ba-4337-ad0c-57ff2fea3581.jpg?v=1772019284","url":"https:\/\/www.ebiohippo.com\/products\/shb-antibody-sh2-domain-containing-adapter-protein-b-bha17135282","provider":"BioHippo","version":"1.0","type":"link"}