{"product_id":"she-antibody-sh2-domain-containing-adapter-protein-e-bha17135755","title":"SHE Antibody \/ SH2 domain-containing adapter protein E","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eSHE Antibody \/ SH2 domain-containing adapter protein E is a anti-SHE Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasmic, Nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e SHE\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, IF, ICC\/IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eSHE antibody detects SH2 domain-containing adapter protein E, a cytoplasmic signaling adaptor that mediates growth factor and cytokine receptor signaling. Encoded by the SHE gene on chromosome 2p13.3, this protein functions downstream of receptor tyrosine kinases and cytokine receptors, facilitating the recruitment of effector molecules involved in cell growth, differentiation, and survival. By coupling phosphorylated receptors to intracellular signaling cascades, SHE helps coordinate pathways such as MAPK\/ERK and PI3K\/AKT.\u003cbr\u003e\u003cbr\u003eSHE contains a central SH2 domain that binds phosphotyrosine motifs on activated receptors and associated kinases. Through its modular architecture, it acts as a scaffold that brings kinases and transcriptional regulators into close proximity, ensuring precise and timely propagation of receptor-initiated signals. SHE interacts with proteins such as EGFR, JAK2, and STAT3, influencing processes from hematopoietic differentiation to epithelial cell proliferation.\u003cbr\u003e\u003cbr\u003eThe SHE antibody is widely used in cell signaling, oncology, and hematology research to study adaptor-mediated signal transduction and receptor dynamics. Western blot analysis identifies a 62 kilodalton band corresponding to SHE, while immunofluorescence shows cytoplasmic and perinuclear localization in stimulated cells. This antibody is essential for dissecting receptor coupling events and understanding how adaptor proteins coordinate cellular responses to growth factors.\u003cbr\u003e\u003cbr\u003eIn cancer and immune regulation, altered SHE expression or phosphorylation disrupts normal signal transduction, leading to abnormal cell proliferation and survival. Overactivation of SHE-associated signaling contributes to tumor progression, while its suppression can impair cytokine responsiveness and differentiation. The SHE antibody provides a sensitive tool for evaluating these effects and monitoring adaptor function in normal and disease contexts.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=SHE - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=SHE - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=SHE - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/SHE - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=SHE+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047303340397,"sku":"FY12853","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_f67ae67c-ef07-4cde-b2d9-5c7518489179.jpg?v=1782237042","url":"https:\/\/www.ebiohippo.com\/products\/she-antibody-sh2-domain-containing-adapter-protein-e-bha17135755","provider":"BioHippo","version":"1.0","type":"link"}