{"product_id":"shmt2-antibody-serine-hydroxymethyltransferase-bha17135307","title":"SHMT2 Antibody \/ Serine hydroxymethyltransferase","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eSHMT2 Antibody \/ Serine hydroxymethyltransferase is a anti-SHMT2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasmic, Nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e SHMT2\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, IF, ICC\/IF, IP, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eThe SHMT2 antibody targets Serine hydroxymethyltransferase, mitochondrial, an enzyme encoded by the SHMT2 gene that catalyzes the reversible conversion of serine and tetrahydrofolate to glycine and 5,10-methylenetetrahydrofolate. This reaction is central to one-carbon metabolism, providing key intermediates for nucleotide synthesis, methylation reactions, and redox regulation. Serine hydroxymethyltransferase, mitochondrial operates within the mitochondrial folate cycle and contributes to biosynthetic and energetic demands in proliferating cells. The SHMT2 antibody provides a reliable reagent for studying one-carbon metabolism, mitochondrial function, and cancer metabolism.\u003cbr\u003e\u003cbr\u003eSerine hydroxymethyltransferase, mitochondrial forms homotetramers that utilize pyridoxal phosphate (PLP) as a cofactor for catalysis. Its activity links amino acid metabolism with folate-mediated one-carbon flux, coordinating mitochondrial and cytosolic metabolic networks. The SHMT2 antibody enables visualization of this enzyme within mitochondria, supporting studies of its spatial regulation and contribution to biosynthetic pathways under nutrient limitation or hypoxic conditions. SHMT2 expression increases in rapidly dividing cells to sustain nucleotide production and redox balance.\u003cbr\u003e\u003cbr\u003eAberrant activation of one-carbon metabolism, including SHMT2 upregulation, is a hallmark of cancer metabolism. Overexpression of Serine hydroxymethyltransferase, mitochondrial enhances proliferation and resistance to oxidative stress by supplying reduced folate cofactors and NADPH. The SHMT2 antibody facilitates detection of this metabolic enzyme in tumor tissues and cell lines, aiding evaluation of its role in tumorigenesis and metabolic adaptation. SHMT2 has been proposed as a potential therapeutic target, as inhibition disrupts nucleotide synthesis and redox homeostasis in cancer cells.\u003cbr\u003e\u003cbr\u003eBeyond oncology, Serine hydroxymethyltransferase, mitochondrial contributes to neural development, mitochondrial respiration, and cellular methylation capacity. It provides one-carbon units necessary for formate generation and DNA methylation reactions that influence epigenetic regulation. The SHMT2 antibody supports research into these metabolic control points, enabling functional analysis of folate cycle dynamics in metabolic disorders, neurodegenerative diseases, and aging.\u003cbr\u003e\u003cbr\u003eThe SHMT2 antibody performs effectively in western blotting, immunofluorescence, and immunohistochemistry, showing clear mitochondrial staining patterns.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=SHMT2 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=SHMT2 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=SHMT2 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/SHMT2 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=SHMT2+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047286956397,"sku":"FY12404","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_41753c30-1eb8-44b5-a5e5-60e70054b304.jpg?v=1772019328","url":"https:\/\/www.ebiohippo.com\/products\/shmt2-antibody-serine-hydroxymethyltransferase-bha17135307","provider":"BioHippo","version":"1.0","type":"link"}