{"product_id":"sigirr-antibody-single-ig-il-1-related-receptor-bha17135271","title":"SIGIRR Antibody \/ Single Ig IL-1-related receptor","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eSIGIRR Antibody \/ Single Ig IL-1-related receptor is a anti-SIGIRR Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e SIGIRR\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, IF, ICC\/IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eThe SIGIRR antibody targets Single immunoglobulin and toll-interleukin 1 receptor (TIR) domain-containing protein, a negative regulator of interleukin and toll-like receptor signaling encoded by the SIGIRR gene. Also known as Toll\/IL-1 receptor 8 (TIR8), this protein modulates inflammatory responses by inhibiting MyD88- and IRAK-mediated signal transduction. Single immunoglobulin and toll-interleukin 1 receptor domain-containing protein acts as a brake on excessive innate immune activation, helping to maintain immune tolerance and prevent tissue damage. The SIGIRR antibody provides researchers with a crucial reagent to study innate immune regulation and inflammation control mechanisms.\u003cbr\u003e\u003cbr\u003eSingle immunoglobulin and toll-interleukin 1 receptor domain-containing protein is a type I transmembrane glycoprotein with a single extracellular immunoglobulin-like domain, a transmembrane segment, and an intracellular TIR domain. Unlike classical toll-like receptors, its cytoplasmic TIR domain lacks certain signaling motifs, rendering it inhibitory. The SIGIRR antibody enables detection of this regulatory receptor in epithelial, endothelial, and immune cells, allowing studies on how it fine-tunes inflammatory signaling.\u003cbr\u003e\u003cbr\u003eFunctionally, SIGIRR interacts with IL-1 receptor and TLR complexes to disrupt adaptor recruitment and downstream kinase activation. It thereby attenuates NF-?B and MAPK pathway activation in response to cytokine or pathogen-associated stimuli. The SIGIRR antibody supports investigations into these signaling mechanisms and helps define how reduced SIGIRR expression contributes to hyperinflammatory states and autoimmune diseases. Mice deficient in this protein exhibit enhanced inflammatory responses and are more susceptible to colitis and sepsis, underscoring its protective role.\u003cbr\u003e\u003cbr\u003eBeyond inflammation, Single immunoglobulin and toll-interleukin 1 receptor domain-containing protein contributes to mucosal homeostasis, microbiota balance, and epithelial integrity. In the gut and lungs, it limits IL-1-driven inflammation and maintains tolerance to commensal organisms. The SIGIRR antibody is used in immunohistochemistry and flow cytometry to evaluate tissue distribution, revealing strong expression on epithelial cells of mucosal surfaces. Aberrant regulation of SIGIRR has been linked to inflammatory bowel disease, asthma, and cancer, where loss of this inhibitory signaling may favor tumor-promoting inflammation.\u003cbr\u003e\u003cbr\u003eThe SIGIRR antibody performs effectively in western blotting, immunofluorescence, and immunohistochemistry. It provides clear membrane and cytoplasmic staining consistent with receptor localization.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=SIGIRR - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=SIGIRR - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=SIGIRR - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/SIGIRR - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=SIGIRR+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047286202733,"sku":"FY12368","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_c82b3b46-aeed-46da-9f67-a338be75c9b2.jpg?v=1782237005","url":"https:\/\/www.ebiohippo.com\/products\/sigirr-antibody-single-ig-il-1-related-receptor-bha17135271","provider":"BioHippo","version":"1.0","type":"link"}