{"product_id":"siglec10-nf-kappa-b-reporter-lentivirus-set-bhv19400269","title":"SIGLEC10\/NFκB Reporter Lentivirus Set","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eSIGLEC10 is a sialic acid-binding immunoglobulin-type lectin receptor expressed on macrophages and other immune cells. It carries inhibitory ITIM motifs and, upon engaging sialylated ligands such as CD24, transmits a suppressive signal that dampens macrophage activation and phagocytosis. This CD24-SIGLEC10 axis functions as a 'don't eat me' checkpoint, and tumors can exploit it to evade immune clearance. The reporter in this set uses NF-κB response elements; NF-κB is an inducible transcription factor that integrates signals from immune receptors to drive expression of inflammatory and activation genes, providing a quantitative readout of how SIGLEC10 engagement modulates downstream signaling.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe SIGLEC10\/NFκB Reporter Lentivirus Set is an immunotherapy reporter system supplied as two vials. A receptor lentivirus constitutively expresses human SIGLEC10 with antibiotic selection, while a reporter lentivirus carries tandem NF-κB response elements driving a dual reporter, secreted Gaussia luciferase together with eGFP or mCherry. Sequential transduction and selection generates a dual-stable cell line for monitoring SIGLEC10-modulated NF-κB activity.\u003c\/p\u003e\n\u003cp\u003eSecreted Gaussia luciferase accumulates in conditioned media, allowing kinetic sampling without cell lysis, while fluorescence supports microscopy and flow cytometry. The VSV-G-pseudotyped particles are purified by PEG precipitation and sucrose gradient centrifugation and transduce difficult-to-transfect cells, including primary and thawed cultures. The system supports macrophage inhibition and immune-evasion assays and screening of agents that target the SIGLEC10 checkpoint.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis 2-vial immunotherapy reporter system consists of a \u003cstrong\u003eVial 1 Receptor Lentivirus\u003c\/strong\u003e encoding human SIGLEC10 under a constitutive promoter with antibiotic selection, and a \u003cstrong\u003eVial 2 Reporter Lentivirus\u003c\/strong\u003e encoding tandem NFAT (or NF-κB) response elements driving a dual reporter (Gaussia Luciferase + eGFP, Gaussia Luciferase + mCherry). Sequential transduction and selection generates a dual-stable effector cell line that responds quantitatively to receptor stimulation with a ratiometric fluorescent + bioluminescent readout.\u003c\/p\u003e\n\u003cp\u003eSecreted Gaussia luciferase (where included) accumulates in conditioned media, enabling kinetic sampling without cell lysis. The combined fluorescent and luminescent outputs allow parallel microscopy-based visualization and plate-reader luminometry from the same cell population — providing assay redundancy and flexibility for potency testing formats compliant with regulatory expectations for cell-based functional assays.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"Gaussia Luciferase + eGFP \/ Puromycin + Blasticidin \/ 2x10^6 per vial","offer_id":53251624763757,"sku":"TRV-0021-SET1","price":1200.0,"currency_code":"USD","in_stock":true},{"title":"Gaussia Luciferase + mCherry \/ Puromycin + Blasticidin \/ 2x10^6 per vial","offer_id":53362031264109,"sku":"TRV-0021-SET2","price":1200.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/Immunotherapy-Reporter-Set-Construct-Diagrams-1_77fc6da9-0be0-4118-b915-07aaf8c743b0.jpg?v=1776934148","url":"https:\/\/www.ebiohippo.com\/products\/siglec10-nf-kappa-b-reporter-lentivirus-set-bhv19400269","provider":"BioHippo","version":"1.0","type":"link"}