{"product_id":"sik1-antibody-salt-inducible-kinase-1-bha17135422","title":"SIK1 Antibody \/ Salt-inducible kinase 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eSIK1 Antibody \/ Salt-inducible kinase 1 is a anti-SIK1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear, cytoplasmic.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e SIK1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC\/IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eSIK1 antibody detects Salt-inducible kinase 1, a serine\/threonine kinase that regulates energy metabolism, stress signaling, and transcriptional responses to hormonal cues. SIK1 belongs to the AMP-activated protein kinase (AMPK) family and is activated by LKB1-mediated phosphorylation. The SIK1 antibody is commonly used to investigate glucose metabolism, circadian rhythm, and stress adaptation in mammalian cells.\u003cbr\u003e\u003cbr\u003eSIK1 is encoded by the SIK1 gene located on human chromosome 21q22.3. The protein is approximately 78 kilodaltons and consists of an N-terminal kinase domain, an autoinhibitory sequence, and a C-terminal region rich in phosphorylation sites. SIK1 modulates transcription by phosphorylating CREB-regulated transcription coactivators (CRTCs), leading to their cytoplasmic retention and suppression of CREB-dependent gene expression.\u003cbr\u003e\u003cbr\u003eThe SIK1 antibody detects a strong 85 kilodalton band by western blot. Under stress or hormonal stimulation, SIK1 undergoes rapid induction and nuclear translocation. It acts as a feedback regulator controlling CREB activity, gluconeogenic gene expression, and circadian rhythm stability.\u003cbr\u003e\u003cbr\u003eFunctionally, SIK1 plays an important role in metabolic homeostasis. It regulates hepatic glucose output, lipid metabolism, and skeletal muscle adaptation to exercise. In the brain, SIK1 contributes to neuronal plasticity and sleep-wake regulation. Dysregulation of SIK1 expression or phosphorylation has been implicated in hypertension, metabolic syndrome, and mood disorders.\u003cbr\u003e\u003cbr\u003eSIK1 is a key mediator of hormonal signaling via cAMP and glucocorticoids. It interacts with 14-3-3 proteins and phosphatases, allowing dynamic control of kinase activity in response to changing energy states.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=SIK1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=SIK1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=SIK1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/SIK1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=SIK1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047291412845,"sku":"FY12519","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_9eae7ce3-9e0b-446a-bb6b-0824ce7989ef.jpg?v=1782237012","url":"https:\/\/www.ebiohippo.com\/products\/sik1-antibody-salt-inducible-kinase-1-bha17135422","provider":"BioHippo","version":"1.0","type":"link"}