{"product_id":"sirpa-nf-kappa-b-reporter-lentivirus-set-bhv19400256","title":"SIRPA\/NFκB Reporter Lentivirus Set","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eSIRPα (SIRPA) is an inhibitory transmembrane receptor expressed predominantly on macrophages and dendritic cells. Its ligand, CD47, is a widely expressed immunoglobulin-superfamily protein that is frequently overexpressed by tumor cells. Engagement of CD47 by SIRPα transmits a \"don't eat me\" signal that suppresses phagocytosis, allowing cancer cells to evade macrophage-mediated clearance. Blocking the CD47-SIRPα axis with antibodies or SIRPα-Fc fusion proteins restores phagocytic activity, making this interaction a major target in cancer immunotherapy. NF-κB is a key transcription factor activated downstream of innate immune receptors, providing a quantitative readout of pathway engagement in this reporter system.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe SIRPA\/NFκB Reporter Lentivirus Set is a two-component immunotherapy reporter system for studying the CD47-SIRPα phagocytosis checkpoint. One lentivirus constitutively expresses the SIRPA receptor; the second carries NF-κB response elements driving a dual reporter of secreted Gaussia luciferase and a fluorescent protein (eGFP or mCherry). Upon receptor engagement, NF-κB activation produces a quantitative luminescent and fluorescent signal. Sequential transduction and selection generate a stable reporter cell line for measuring the potency of SIRPα-blocking antibodies or SIRPα-Fc in \"don't eat me\" phagocytosis-inhibition assays. Secreted Gaussia luciferase enables non-destructive kinetic sampling from conditioned media. Supplied as high-titer, VSV-G-pseudotyped third-generation particles purified by PEG precipitation and sucrose gradient centrifugation, suitable for primary and thawed cells.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis 2-vial immunotherapy reporter system consists of a \u003cstrong\u003eVial 1 Receptor Lentivirus\u003c\/strong\u003e encoding human Don't Eat Me' Assay under a constitutive promoter with antibiotic selection, and a \u003cstrong\u003eVial 2 Reporter Lentivirus\u003c\/strong\u003e encoding tandem NFAT (or NF-κB) response elements driving a dual reporter (Gaussia Luciferase + eGFP, Gaussia Luciferase + mCherry). Sequential transduction and selection generates a dual-stable effector cell line that responds quantitatively to receptor stimulation with a ratiometric fluorescent + bioluminescent readout.\u003c\/p\u003e\n\u003cp\u003eSecreted Gaussia luciferase (where included) accumulates in conditioned media, enabling kinetic sampling without cell lysis. The combined fluorescent and luminescent outputs allow parallel microscopy-based visualization and plate-reader luminometry from the same cell population — providing assay redundancy and flexibility for potency testing formats compliant with regulatory expectations for cell-based functional assays.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"Gaussia Luciferase + eGFP \/ Puromycin + Blasticidin \/ 2x10^6 per vial","offer_id":53251619029357,"sku":"TRV-0007-SET1","price":1200.0,"currency_code":"USD","in_stock":true},{"title":"Gaussia Luciferase + mCherry \/ Puromycin + Blasticidin \/ 2x10^6 per vial","offer_id":53362031526253,"sku":"TRV-0007-SET2","price":1200.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/Immunotherapy-Reporter-Set-Construct-Diagrams-1.jpg?v=1776934137","url":"https:\/\/www.ebiohippo.com\/products\/sirpa-nf-kappa-b-reporter-lentivirus-set-bhv19400256","provider":"BioHippo","version":"1.0","type":"link"}