{"product_id":"ska3-antibody-spindle-and-kinetochore-associated-complex-subunit-3-c13orf3-bha17136150","title":"SKA3 Antibody \/ Spindle and kinetochore associated complex subunit 3 \/ C13orf3","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eSKA3 Antibody \/ Spindle and kinetochore associated complex subunit 3 \/ C13orf3 is a anti-SKA3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), ELISA with listed reactivity in Human. Reported localization: Cytoplasm.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e SKA3\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eSKA3 antibody detects Spindle and kinetochore associated complex subunit 3, a mitotic protein required for chromosome segregation and kinetochore-microtubule attachment. The UniProt recommended name is Spindle and kinetochore associated complex subunit 3 (SKA3). Also known as C13orf3, this protein forms part of the SKA complex, along with SKA1 and SKA2, which stabilizes kinetochore-spindle microtubule interactions during metaphase and anaphase.\u003cbr\u003e\u003cbr\u003eFunctionally, SKA3 antibody identifies a 412-amino-acid cytoplasmic and kinetochore-localized protein that binds microtubules and interacts with the Ndc80 complex to anchor chromosomes to spindle fibers. The SKA complex is recruited to kinetochores in a microtubule-dependent manner and facilitates the coupling of depolymerizing microtubules to chromosome movement. SKA3 plays a crucial role in maintaining mitotic fidelity and preventing chromosome missegregation. It is also required for silencing the spindle assembly checkpoint once proper attachment is achieved, allowing progression into anaphase.\u003cbr\u003e\u003cbr\u003eThe SKA3 gene is located on chromosome 13q12.13 and is expressed in proliferating cells, with elevated expression during the G2\/M phase of the cell cycle. Its transcription is regulated by E2F family transcription factors, linking SKA3 expression to cell cycle progression and mitotic entry.\u003cbr\u003e\u003cbr\u003ePathologically, dysregulation of SKA3 contributes to chromosomal instability, aneuploidy, and cancer. Overexpression has been observed in breast, liver, and lung cancers, where it promotes uncontrolled proliferation and correlates with poor prognosis. Loss of SKA3 function disrupts kinetochore attachment and induces mitotic arrest. Research using SKA3 antibody supports studies in mitosis, spindle checkpoint control, and cancer biology.\u003cbr\u003e\u003cbr\u003eSKA3 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect mitotic spindle-associated proteins. \u003cbr\u003e\u003cbr\u003eStructurally, Spindle and kinetochore associated complex subunit 3 contains coiled-coil domains that mediate oligomerization and interaction with SKA1\/SKA2. Its C-terminal region binds directly to microtubules, while its N-terminal domain interacts with kinetochore complexes. This modular organization enables SKA3 to function as a mechanical linker between depolymerizing microtubules and kinetochores. This antibody allows precise analysis of SKA3's role in mitotic regulation and chromosomal stability.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=SKA3 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=SKA3 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=SKA3 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/SKA3 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=SKA3+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047319822701,"sku":"FY13248","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_7e01dae9-bc10-4ae5-ad09-7cd5175c966a.jpg?v=1782237074","url":"https:\/\/www.ebiohippo.com\/products\/ska3-antibody-spindle-and-kinetochore-associated-complex-subunit-3-c13orf3-bha17136150","provider":"BioHippo","version":"1.0","type":"link"}