{"product_id":"skf-96365-hydrochloride-bhb21300323","title":"SKF 96365 hydrochloride","description":"\u003ch3\u003eProduct details\u003c\/h3\u003e \u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eChemical name:\u003c\/strong\u003e 1-[2-(4-methoxyphenyl)-2-[3-(4-methoxyphenyl)propoxy]ethyl]imidazole;hydrochloride.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCAS number:\u003c\/strong\u003e 130495-35-1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMolecular formula:\u003c\/strong\u003e C22H27ClN2O3.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurity:\u003c\/strong\u003e \u0026gt;98%\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eConcentration:\u003c\/strong\u003e 1-100 µM.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eForm:\u003c\/strong\u003e Lyophilized powder.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSolubility:\u003c\/strong\u003e 100 mM in H2O. Centrifuge all product preparations before use (10 000 x g for 1 min).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReconstitution:\u003c\/strong\u003e 100 mM in H2O. Centrifuge all product preparations before use (10 000 x g for 1 min).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eShipping:\u003c\/strong\u003e Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e CaV3 channels, TRPC, STIM1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSource:\u003c\/strong\u003e Synthetic\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eActivity:\u003c\/strong\u003e SKF 96365 is an Inhibitor of TRPC channels and inhibitor of T-type calcium channels with an IC50 560 nM for CaV3.1 channels1.\u003c\/li\u003e\n\u003c\/ul\u003e \u003ch3\u003eScientific background\u003c\/h3\u003e \u003cp\u003eSKF 96365 hydrochloride is a synthetic blocker of CaV3 (T-type) voltage gated calcium channels and of TRPC channels. In addition, it has a blocking effect on potassium channels. SKF also inhibits the calcium sensor STIM1 which enables Store-operated Ca2+ entry (SOCE). SKF has an effective concentration of 1-100 µM and an IC50 of 560 nM for CaV3.1 channel. SKF is used to identify CaV3 and TRPC channels in many cell types. In an experiment examining the effect of SKF on CaV3 conductivity, perfusion of 1 µM of SKF reversibly inhibited 86.3±0.1% of CaV3.1 currents generated in HEK293 cells. Maximum inhibition was reached within 6-7 minutes. Application of larger concentrations of SKF completely abolished CaV3.1 channel currents. SKF blocks the CaV3.1 channel in a tonic manner indicating it interacts with the channel regardless of its functional state, unlike other CaV channel blockers. In the same experiment SKF also abolished calcium currents in other types of CaV channels such as P\/Q-type and N-type.Blockade of these channels was only partially reversible due to possible irreversible binding of or longer washing time required. In addition, SKF blocks L-type calcium channels but in a much slower manner than other calcium channels.\u003c\/p\u003e \u003cp\u003e\u003cstrong\u003eLead time:\u003c\/strong\u003e 1-2 Business Days\u003c\/p\u003e \u003cp\u003e\u003cstrong\u003eCountry of origin:\u003c\/strong\u003e Israel\/IL\u003c\/p\u003e \u003ch3\u003eApplications key\u003c\/h3\u003e \u003cp\u003eApplication key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.\u003c\/p\u003e \u003cp\u003e\u003cstrong\u003eBioassay tested:\u003c\/strong\u003e Yes\u003c\/p\u003e","brand":"Alomone Labs","offers":[{"title":"10 mg \/ 1","offer_id":53249714159981,"sku":"S-175-10MG-1","price":132.0,"currency_code":"USD","in_stock":true},{"title":"25 mg \/ 1","offer_id":53311576965485,"sku":"S-175-25MG-1","price":297.0,"currency_code":"USD","in_stock":true},{"title":"5 mg \/ 1","offer_id":53311576998253,"sku":"S-175-5MG-1","price":92.0,"currency_code":"USD","in_stock":true},{"title":"50 mg \/ 1","offer_id":53311577031021,"sku":"S-175-50MG-1","price":537.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/S-175_gr.gif?v=1778544452","url":"https:\/\/www.ebiohippo.com\/products\/skf-96365-hydrochloride-bhb21300323","provider":"BioHippo","version":"1.0","type":"link"}