{"product_id":"slc25a12-antibody-aralar1-bha17135886","title":"SLC25A12 Antibody \/ ARALAR1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eSLC25A12 Antibody \/ ARALAR1 is a anti-SLC25A12 Rabbit antibody Recombinant Rabbit Monoclonal clone 30S67 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunoprecipitation (IP) with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e SLC25A12\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 30S67, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IP\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eSLC25A12 antibody detects Calcium binding mitochondrial carrier protein Aralar2, encoded by the SLC25A12 gene. This protein belongs to the solute carrier family 25, which consists of mitochondrial carriers responsible for transporting metabolites across the inner mitochondrial membrane. Aralar2 functions primarily as an aspartate glutamate carrier, playing a critical role in the malate aspartate shuttle, which transfers reducing equivalents from the cytosol into the mitochondria. SLC25A12 antibody provides researchers with a reliable reagent for studying mitochondrial metabolism, neurodevelopment, and energy regulation.\u003cbr\u003e\u003cbr\u003eCalcium binding mitochondrial carrier protein Aralar2 is highly expressed in the brain, heart, and skeletal muscle, reflecting its role in tissues with high energy demand. The protein's activity is regulated by calcium ions, which bind to EF hand domains in its N terminal region. When detected with SLC25A12 antibody, Aralar2 has been shown to facilitate the exchange of cytosolic glutamate for mitochondrial aspartate, linking amino acid metabolism with cellular energy pathways. This shuttle activity supports processes such as oxidative phosphorylation, urea cycle function, and neurotransmitter synthesis.\u003cbr\u003e\u003cbr\u003eMutations in SLC25A12 have been associated with neurodevelopmental disorders, including a subtype of early infantile epileptic encephalopathy. Patients with pathogenic variants present with seizures, developmental delay, and structural brain abnormalities. Studies using SLC25A12 antibody have confirmed that loss of Aralar2 disrupts neuronal energy metabolism and neurotransmitter balance. Beyond rare genetic disease, altered expression of this protein has been implicated in autism spectrum disorders, highlighting its importance in brain development and synaptic signaling.\u003cbr\u003e\u003cbr\u003eResearch has also connected Aralar2 to broader metabolic functions, including regulation of insulin secretion and lipid metabolism. SLC25A12 antibody has been used to demonstrate its presence in pancreatic islets, where glutamate aspartate exchange influences insulin granule release. In liver and muscle, changes in Aralar2 expression affect metabolic flexibility, making it relevant to diabetes and obesity studies. These findings highlight the wide physiological significance of Calcium binding mitochondrial carrier protein Aralar2 beyond the nervous system.\u003cbr\u003e\u003cbr\u003eSLC25A12 antibody is widely used in western blotting, immunohistochemistry, and immunofluorescence. Western blotting confirms expression in brain and muscle lysates, while immunohistochemistry demonstrates its localization to mitochondria within neuronal tissue. Immunofluorescence assays further confirm subcellular localization, colocalizing Aralar2 with mitochondrial markers. Functional studies using SLC25A12 antibody provide insights into how altered transporter activity affects metabolic pathways under physiological and pathological conditions.\u003cbr\u003e\u003cbr\u003eBy providing validated SLC25A12 antibody reagents,\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=SLC25A12 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=SLC25A12 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=SLC25A12 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/SLC25A12 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=SLC25A12+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047308517741,"sku":"FY12984","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_3f6c7331-4ebd-4e2c-b242-71f9a8c6574b.jpg?v=1782237071","url":"https:\/\/www.ebiohippo.com\/products\/slc25a12-antibody-aralar1-bha17135886","provider":"BioHippo","version":"1.0","type":"link"}