{"product_id":"slc7a7-antibody-solute-carrier-family-7-member-7-bha17136184","title":"SLC7A7 Antibody \/ Solute carrier family 7 member 7","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eSLC7A7 Antibody \/ Solute carrier family 7 member 7 is a anti-SLC7A7 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cell membrane.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e SLC7A7\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, IF, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eSLC7A7 antibody recognizes Solute carrier family 7 member 7, also known as y+LAT1, a light chain subunit of the heteromeric amino acid transporter system y+L. Encoded by the SLC7A7 gene on chromosome 14q11.2, this protein forms a heterodimer with the heavy chain 4F2 cell surface antigen (SLC3A2\/CD98) to mediate sodium-independent transport of cationic amino acids such as lysine and arginine, and exchange with neutral amino acids in the presence of sodium ions. SLC7A7 plays a vital role in amino acid homeostasis, particularly in epithelial cells of the intestine, kidney, and macrophages.\u003cbr\u003e\u003cbr\u003eDefects in SLC7A7 cause lysinuric protein intolerance (LPI), a rare inherited metabolic disorder characterized by impaired transport of cationic amino acids leading to protein intolerance, hyperammonemia, and growth retardation. In patients with LPI, mutations in SLC7A7 disrupt transporter assembly or function, resulting in abnormal accumulation of amino acids in urine and reduced absorption in the intestine and kidney. Research on SLC7A7 has advanced understanding of amino acid sensing pathways and their link to mTOR signaling, autophagy, and immune regulation.\u003cbr\u003e\u003cbr\u003eAt the cellular level, SLC7A7 is localized predominantly at the plasma membrane of polarized epithelial cells, where it supports nutrient absorption and nitrogen balance. The protein also contributes to macrophage activation and cytokine production, influencing inflammatory responses. Its expression is regulated by transcription factors including ATF4 and by nutrient availability, reflecting its central role in amino acid-responsive metabolic control. Experimental models indicate that SLC7A7 deficiency may lead to mitochondrial dysfunction and oxidative stress, providing insight into the metabolic complications of LPI.\u003cbr\u003e\u003cbr\u003eImmunohistochemical analysis using SLC7A7 antibody shows staining in renal tubular epithelium, intestinal villi, and hepatocytes. It serves as a useful biomarker for investigating amino acid transporter function, metabolic disease mechanisms, and nutrient-dependent signaling. The SLC7A7 antibody from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=SLC7A7 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=SLC7A7 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=SLC7A7 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/SLC7A7 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=SLC7A7+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047320904045,"sku":"FY13282","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_266c93f4-4b0e-4a6f-82b5-f011c3906f70.jpg?v=1782237083","url":"https:\/\/www.ebiohippo.com\/products\/slc7a7-antibody-solute-carrier-family-7-member-7-bha17136184","provider":"BioHippo","version":"1.0","type":"link"}