{"product_id":"slc9a3-antibody-sodium-hydrogen-exchanger-3-bha17135516","title":"SLC9A3 Antibody \/ Sodium\/hydrogen exchanger 3","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eSLC9A3 Antibody \/ Sodium\/hydrogen exchanger 3 is a anti-SLC9A3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), ELISA with listed reactivity in Human, Mouse.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e SLC9A3\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, IP, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eSLC9A3 antibody detects Sodium\/hydrogen exchanger 3, a membrane transporter that regulates intracellular pH and sodium absorption in epithelial tissues. SLC9A3 plays a vital role in electrolyte balance, acid-base homeostasis, and fluid reabsorption in the kidney and intestine. The SLC9A3 antibody is widely used in renal physiology, gastrointestinal, and cell signaling research to study ion transport mechanisms and epithelial function.\u003cbr\u003e\u003cbr\u003eSLC9A3 is encoded by the SLC9A3 gene located on human chromosome 5p15.33. The protein is approximately 834 amino acids long and belongs to the solute carrier 9 family of sodium\/hydrogen exchangers (NHEs). It is predominantly expressed in the apical membrane of renal proximal tubule and intestinal epithelial cells, where it exchanges intracellular hydrogen ions for extracellular sodium ions, helping to regulate pH and volume.\u003cbr\u003e\u003cbr\u003eThe SLC9A3 antibody detects a 90 kilodalton band by western blot and shows apical membrane staining in epithelial tissues under immunofluorescence microscopy. Through its role in sodium absorption, SLC9A3 contributes to systemic blood pressure control and fluid balance. Regulation of SLC9A3 activity is mediated by phosphorylation and interaction with scaffold proteins such as NHERF1 and ezrin, linking it to hormonal control by angiotensin II and parathyroid hormone.\u003cbr\u003e\u003cbr\u003eMutations in SLC9A3 cause congenital secretory diarrhea and male infertility due to defective sodium transport and fluid absorption. In the kidney, SLC9A3 dysfunction results in impaired bicarbonate reabsorption and metabolic acidosis. Its regulation is critical for maintaining acid-base equilibrium, and its altered expression has been linked to cystic fibrosis and inflammatory bowel disease.\u003cbr\u003e\u003cbr\u003eAs an essential component of epithelial ion transport, SLC9A3 integrates metabolic, hormonal, and mechanical stimuli to maintain homeostasis.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=SLC9A3 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=SLC9A3 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=SLC9A3 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/SLC9A3 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=SLC9A3+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047294067053,"sku":"FY12613","price":449.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/www.ebiohippo.com\/products\/slc9a3-antibody-sodium-hydrogen-exchanger-3-bha17135516","provider":"BioHippo","version":"1.0","type":"link"}