{"product_id":"slc9a8-antibody-sodium-hydrogen-exchanger-8-nhe8-bha17136027","title":"SLC9A8 Antibody \/ Sodium\/hydrogen exchanger 8 \/ NHE8","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eSLC9A8 Antibody \/ Sodium\/hydrogen exchanger 8 \/ NHE8 is a anti-SLC9A8 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e SLC9A8\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eSLC9A8 antibody detects Sodium\/hydrogen exchanger 8, a membrane transporter involved in intracellular pH regulation and sodium ion homeostasis. The UniProt recommended name is Sodium\/hydrogen exchanger 8 (SLC9A8). Also known as NHE8, this transporter belongs to the solute carrier family 9 and mediates electroneutral exchange of sodium and hydrogen ions across cellular membranes.\u003cbr\u003e\u003cbr\u003eFunctionally, SLC9A8 antibody identifies a 576-amino-acid integral membrane protein localized primarily to the trans-Golgi network and apical plasma membrane. SLC9A8 contributes to luminal pH control in endosomes and the Golgi apparatus, influencing protein sorting and vesicular trafficking. It also maintains intestinal epithelial ion balance and supports mucosal barrier function.\u003cbr\u003e\u003cbr\u003eThe SLC9A8 gene is located on chromosome 20q13.13 and is widely expressed, with highest levels in kidney, intestine, and testis. NHE8 activity complements other sodium\/hydrogen exchangers such as NHE3, particularly during development and epithelial differentiation. Its regulation ensures proper acid-base balance and cellular volume control.\u003cbr\u003e\u003cbr\u003ePathologically, loss of SLC9A8 function has been associated with intestinal dysfunction, renal tubular acidosis, and retinal degeneration. In epithelial cells, SLC9A8 deficiency impairs vesicular acidification and protein trafficking. Research using SLC9A8 antibody aids studies in membrane transport, epithelial physiology, and cellular pH regulation.\u003cbr\u003e\u003cbr\u003eSLC9A8 antibody is validated for western blotting, immunohistochemistry, and immunofluorescence to detect sodium\/hydrogen exchangers and intracellular transport proteins. \u003cbr\u003e\u003cbr\u003eStructurally, Sodium\/hydrogen exchanger 8 contains multiple transmembrane helices forming an ion translocation pathway and a cytoplasmic regulatory domain modulated by intracellular pH and signaling kinases. This antibody supports investigation of SLC9A8's function in vesicular trafficking and epithelial ion regulation.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=SLC9A8 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=SLC9A8 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=SLC9A8 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/SLC9A8 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=SLC9A8+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047313203565,"sku":"FY13125","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_973891ad-d7ce-40df-b137-450f0b5d511c.jpg?v=1782237093","url":"https:\/\/www.ebiohippo.com\/products\/slc9a8-antibody-sodium-hydrogen-exchanger-8-nhe8-bha17136027","provider":"BioHippo","version":"1.0","type":"link"}