{"product_id":"slf2-antibody-smc5-smc6-complex-localization-factor-protein-2-bha17135293","title":"SLF2 Antibody \/ SMC5-SMC6 complex localization factor protein 2","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eSLF2 Antibody \/ SMC5-SMC6 complex localization factor protein 2 is a anti-SLF2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e SLF2\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC\/IF, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eThe SLF2 antibody targets SMC5-SMC6 complex localization factor 2, a nuclear protein encoded by the SLF2 gene that plays an essential role in DNA replication and repair. Also known as FAM178A, this protein helps recruit the SMC5\/6 complex to chromatin following DNA damage, thereby promoting genome stability and replication fork recovery. SMC5-SMC6 complex localization factor 2 ensures proper DNA repair and cell cycle progression under genotoxic stress. The SLF2 antibody provides researchers with a precise tool to study DNA damage response pathways and chromosomal maintenance mechanisms.\u003cbr\u003e\u003cbr\u003eSMC5-SMC6 complex localization factor 2 operates in coordination with its partner SLF1 to anchor the SMC5\/6 complex at damaged replication forks. This localization facilitates homologous recombination repair and prevents collapse of stalled forks. The SLF2 antibody enables visualization of this critical repair factor in nuclear extracts and fixed cells, supporting detailed analysis of DNA repair kinetics and checkpoint activation. Its expression increases during replication stress and following exposure to DNA-damaging agents such as UV or ionizing radiation.\u003cbr\u003e\u003cbr\u003eDisruption of SLF2 function compromises genomic integrity, leading to accumulation of DNA breaks, chromosomal abnormalities, and replication defects. Such genomic instability is a hallmark of cancer and aging. The SLF2 antibody aids in assessing how loss of SMC5-SMC6 recruitment affects DNA repair fidelity and cellular survival. In experimental models, depletion of SMC5-SMC6 complex localization factor 2 sensitizes cells to replication inhibitors, emphasizing its protective role during S phase.\u003cbr\u003e\u003cbr\u003eBeyond replication stress response, SMC5-SMC6 complex localization factor 2 participates in maintaining telomere integrity and regulating chromatin compaction. The SLF2 antibody can be used to explore these broader nuclear processes, providing insight into how SMC5\/6 and associated proteins orchestrate chromosomal architecture. Altered SLF2 expression has been implicated in tumorigenesis and therapy resistance, highlighting its relevance in cancer biology.\u003cbr\u003e\u003cbr\u003eThe SLF2 antibody performs effectively in western blotting, immunofluorescence, and immunohistochemistry, showing strong nuclear localization consistent with its DNA repair function.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=SLF2 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=SLF2 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=SLF2 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/SLF2 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=SLF2+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047287185773,"sku":"FY12390","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_d021fb61-a25f-43a0-a236-c3588e54581a.jpg?v=1782237003","url":"https:\/\/www.ebiohippo.com\/products\/slf2-antibody-smc5-smc6-complex-localization-factor-protein-2-bha17135293","provider":"BioHippo","version":"1.0","type":"link"}