{"product_id":"slirp-antibody-sra-stem-loop-interacting-rna-binding-protein-bha17135514","title":"SLIRP Antibody \/ SRA stem-loop interacting RNA-binding protein","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eSLIRP Antibody \/ SRA stem-loop interacting RNA-binding protein is a anti-SLIRP Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow cytometry (FACS), ELISA with listed reactivity in Human. Reported localization: Mitochondria (predominant), nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e SLIRP\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, IF, ICC\/IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eSLIRP antibody detects SRA stem-loop interacting RNA-binding protein, a mitochondrial RNA-binding protein that modulates gene expression, oxidative phosphorylation, and steroid receptor signaling. SLIRP stabilizes mitochondrial mRNAs and participates in post-transcriptional regulation of respiratory chain components. The SLIRP antibody is widely used in mitochondrial biology and molecular endocrinology to study RNA regulation, energy metabolism, and hormone receptor activity.\u003cbr\u003e\u003cbr\u003eSLIRP is encoded by the SLIRP gene located on human chromosome 14q13.3. The protein is approximately 111 amino acids long and contains an RNA recognition motif responsible for binding SRA (steroid receptor RNA activator) and mitochondrial transcripts. SLIRP localizes primarily to the mitochondrial matrix but also associates with nuclear hormone receptors, linking energy metabolism with transcriptional regulation.\u003cbr\u003e\u003cbr\u003eThe SLIRP antibody detects a 12 kilodalton band by western blot and exhibits mitochondrial and nuclear staining under immunofluorescence. SLIRP forms complexes with LRPPRC, ensuring stabilization and translation of mitochondrial mRNAs encoding respiratory complex subunits. Loss of SLIRP leads to reduced mitochondrial translation, impaired oxidative phosphorylation, and decreased ATP production.\u003cbr\u003e\u003cbr\u003eBeyond mitochondrial function, SLIRP interacts with steroid receptors such as estrogen receptor alpha and glucocorticoid receptor, modulating their transcriptional activity. By coupling nuclear hormone signaling with mitochondrial bioenergetics, SLIRP coordinates cellular responses to hormonal and metabolic cues. Dysregulation of SLIRP expression contributes to metabolic disorders, neurodegeneration, and certain cancers where mitochondrial dysfunction plays a role.\u003cbr\u003e\u003cbr\u003eBecause SLIRP integrates transcriptional control and mitochondrial energy metabolism, it represents a valuable target for understanding gene expression coordination and metabolic adaptation.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=SLIRP - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=SLIRP - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=SLIRP - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/SLIRP - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=SLIRP+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047294427501,"sku":"FY12611","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_900b2bb5-3a64-4f7b-b0c7-236a02eb0b0d.jpg?v=1782237048","url":"https:\/\/www.ebiohippo.com\/products\/slirp-antibody-sra-stem-loop-interacting-rna-binding-protein-bha17135514","provider":"BioHippo","version":"1.0","type":"link"}