{"product_id":"slx4-antibody-structure-specific-endonuclease-subunit-slx4-bha17135258","title":"SLX4 Antibody \/ Structure-specific endonuclease subunit SLX4","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eSLX4 Antibody \/ Structure-specific endonuclease subunit SLX4 is a anti-SLX4 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA with listed reactivity in Human. Reported localization: Nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e SLX4\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e IHC, IF, ICC\/IF, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eThe SLX4 antibody targets Structure-specific endonuclease subunit SLX4, a multifunctional DNA repair scaffold protein encoded by the SLX4 gene. SLX4 coordinates several endonucleases, including SLX1, MUS81-EME1, and XPF-ERCC1, to resolve DNA intermediates that arise during replication, recombination, and repair. Through its interaction network, Structure-specific endonuclease subunit SLX4 ensures proper processing of Holliday junctions, interstrand crosslinks, and stalled replication forks. The SLX4 antibody provides researchers with an essential reagent to study how this protein maintains genomic stability and prevents chromosomal abnormalities.\u003cbr\u003e\u003cbr\u003eSLX4 serves as a platform that organizes structure-specific nucleases into a regulated complex. It promotes timely incision and resolution of DNA structures to prevent recombination defects and chromosome missegregation. The SLX4 antibody is particularly useful for investigating DNA damage responses following genotoxic stress, allowing visualization of nuclear foci formation at replication and repair sites. By detecting SLX4 expression and localization, researchers can monitor its participation in maintaining DNA replication fidelity and chromosomal stability.\u003cbr\u003e\u003cbr\u003eMutations in SLX4 cause Fanconi anemia complementation group P (FANCP), a rare hereditary disorder characterized by genomic instability, bone marrow failure, and cancer predisposition. These pathogenic variants disrupt SLX4's ability to interact with partner nucleases, compromising crosslink repair. The SLX4 antibody supports studies of Fanconi anemia mechanisms and helps identify defects in DNA repair pathways that arise from SLX4 dysfunction. Analysis of Structure-specific endonuclease subunit SLX4 expression also provides insight into its regulatory interactions with checkpoint proteins such as ATR and FANCD2.\u003cbr\u003e\u003cbr\u003eIn addition to its nuclear repair functions, SLX4 has roles in telomere maintenance, homologous recombination, and mitotic checkpoint regulation. The SLX4 antibody enables mapping of these diverse activities by revealing subcellular localization dynamics during the cell cycle. Western blot and immunofluorescence experiments with this antibody show how SLX4 expression fluctuates in S-phase and peaks during mitosis, correlating with its role in resolving replication intermediates.\u003cbr\u003e\u003cbr\u003eBecause genomic stability is a cornerstone of cancer prevention, SLX4 has gained increasing relevance in oncology. Altered expression levels may influence tumor sensitivity to DNA-damaging agents such as cisplatin. The SLX4 antibody supplied by\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=SLX4 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=SLX4 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=SLX4 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/SLX4 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=SLX4+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047285743981,"sku":"FY12355","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_d5d9be15-87f8-4a1d-b473-948d9c298618.jpg?v=1782237008","url":"https:\/\/www.ebiohippo.com\/products\/slx4-antibody-structure-specific-endonuclease-subunit-slx4-bha17135258","provider":"BioHippo","version":"1.0","type":"link"}