{"product_id":"smad2-3-reporter-lentivirus-bhv19400012","title":"SMAD2\/3 Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eSMAD2 and SMAD3 are receptor-regulated SMAD proteins that transduce signals from the transforming growth factor beta (TGF-beta) family of cytokines. When TGF-beta ligands engage their type I and type II serine\/threonine kinase receptors, SMAD2 and SMAD3 are phosphorylated, partner with the common mediator SMAD4, and translocate to the nucleus to regulate transcription of target genes. This pathway controls cell proliferation, differentiation, extracellular matrix production, immune regulation, and embryonic patterning. TGF-beta\/SMAD2\/3 signaling is central to wound healing and tissue homeostasis, and its dysregulation contributes to fibrosis, immune disorders, and cancer, where the pathway can act as either a tumor suppressor or a promoter of progression depending on context.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe SMAD2\/3 Reporter Lentivirus is a transcription factor reporter system that provides a sensitive fluorescent or luminescent readout of TGF-beta pathway activation in transduced cells. The construct places a reporter gene (firefly luciferase, GFP, RFP, Renilla luciferase, or SEAP) under the control of tandem SMAD2\/3 response elements coupled to a minimal promoter and an optimized upstream enhancer that maximizes signal-to-noise. A constitutive drug selection marker (Blasticidin or Puromycin) enables generation of stable polyclonal reporter cell lines. Stable lentiviral integration delivers consistent reporter expression suitable for fluorescence microscopy, flow cytometry, or luminometry. Supplied as high-titer particles purified by PEG precipitation and sucrose gradient centrifugation, it is well suited to studying SMAD2\/3 and TGF-beta signaling in primary and difficult-to-transfect cells.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a Firefly Luc, GFP, Luc, Renilla Luc, RFP, SEAP reporter gene under the control of tandem consensus response elements specific for the TGF-b signaling pathway transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251625451885,"sku":"LTV-0013-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53310892605805,"sku":"LTV-0013-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Puromycin \/ 2x10^6","offer_id":53310892638573,"sku":"LTV-0013-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Blasticidin \/ 2x10^6","offer_id":53310892671341,"sku":"LTV-0013-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53310892704109,"sku":"LTV-0013-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53310892736877,"sku":"LTV-0013-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"SEAP \/ Puromycin \/ 2x10^6","offer_id":53310892769645,"sku":"LTV-0013-7S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"SEAP \/ Blasticidin \/ 2x10^6","offer_id":53310892802413,"sku":"LTV-0013-8S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Renilla Luc \/ Blasticidin \/ 2x10^6","offer_id":53310892835181,"sku":"LTV-0013-4SIC","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/TF-Reporter-Vector-circular_a0614034-8f81-4665-93ee-0eb0e47ba2a0.jpg?v=1776934580","url":"https:\/\/www.ebiohippo.com\/products\/smad2-3-reporter-lentivirus-bhv19400012","provider":"BioHippo","version":"1.0","type":"link"}