{"product_id":"smap2-antibody-small-arfgap-2-bha17135976","title":"SMAP2 Antibody \/ Small ArfGAP 2","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eSMAP2 Antibody \/ Small ArfGAP 2 is a anti-SMAP2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunoprecipitation (IP), Flow cytometry (FACS), ELISA with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e SMAP2\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IP, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eSMAP2 antibody detects Small ArfGAP 2, an ADP-ribosylation factor GTPase-activating protein that regulates membrane trafficking and clathrin-mediated endocytosis. The UniProt recommended name is Small ArfGAP 2 (SMAP2). This cytosolic protein functions as a regulator of ARF family small GTPases, controlling vesicle formation and cargo sorting at the trans-Golgi network and plasma membrane.\u003cbr\u003e\u003cbr\u003eFunctionally, SMAP2 antibody identifies a 429-amino-acid protein containing an ArfGAP domain and a C-terminal clathrin-binding region. SMAP2 stimulates GTP hydrolysis on ARF1 and ARF6, facilitating vesicle uncoating and recycling. It plays a key role in synaptic vesicle turnover, receptor endocytosis, and intracellular trafficking, ensuring efficient communication between organelles.\u003cbr\u003e\u003cbr\u003eThe SMAP2 gene, located on chromosome 1p34.1, is widely expressed in brain, heart, and other tissues with active vesicular transport. In neurons, SMAP2 localizes to presynaptic terminals, where it interacts with clathrin and adaptor proteins to regulate synaptic vesicle cycling. Its activity coordinates endocytosis and exocytosis, maintaining synaptic plasticity and receptor availability.\u003cbr\u003e\u003cbr\u003ePathologically, aberrant SMAP2 expression or dysfunction may contribute to neurological disorders, including synaptic transmission defects and neurodevelopmental abnormalities. Its regulatory role in membrane dynamics also links it to cancer cell migration and secretion. Research with SMAP2 antibody provides insight into ARF signaling, vesicle recycling, and cellular communication mechanisms.\u003cbr\u003e\u003cbr\u003eSMAP2 antibody is suitable for western blotting, immunocytochemistry, and immunoprecipitation to detect SMAP2 in cultured cells and tissues. \u003cbr\u003e\u003cbr\u003eStructurally, SMAP2 contains an N-terminal ArfGAP catalytic domain that interacts with GTP-bound ARF, accelerating GTP hydrolysis, and a C-terminal region that binds clathrin and adaptor proteins. This modular structure allows SMAP2 to act as a molecular bridge between vesicle formation and signaling regulation. This antibody aids in understanding SMAP2�s contribution to intracellular trafficking and membrane remodeling.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=SMAP2 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=SMAP2 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=SMAP2 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/SMAP2 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=SMAP2+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047311237485,"sku":"FY13074","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_3d8b333c-d2bd-4b66-ba0d-e63c90962f82.jpg?v=1782237082","url":"https:\/\/www.ebiohippo.com\/products\/smap2-antibody-small-arfgap-2-bha17135976","provider":"BioHippo","version":"1.0","type":"link"}