{"product_id":"smarca4-antibody-bha10541126","title":"SMARCA4 Antibody","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThis is a polyclonal anti-SMARCA4 antibody raised in Rabbit, with confirmed utility in WB, ELISA. It is designed to detect SMARCA4 protein in Human, Mouse, Rat and supports researchers working in neuroscience contexts where consistent antibody performance is required.\u003c\/p\u003e\n\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunogen:\u003c\/strong\u003e Synthesized peptide derived from the C-terminal region of Human Brg-1. — the immunizing antigen determines the epitope region; confirm epitope compatibility with sample preparation and expected post-translational modifications.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHost species (Rabbit):\u003c\/strong\u003e Requires anti-rabbit-IgG secondary reagents. Use matched secondaries to avoid no-signal or cross-reactivity issues.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePolyclonal format:\u003c\/strong\u003e Recognizes multiple epitopes, providing robust signal across varied preparations and species variants. Inherent lot-to-lot variability requires appropriate controls.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIsotype (IgG):\u003c\/strong\u003e Compatible with standard Protein A\/G purification and widely supported by secondary reagents. Include an isotype-matched control at equivalent concentration.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurification (Antigen affinity purification):\u003c\/strong\u003e Enriches for specific immunoglobulin classes, reducing non-specific populations and improving signal-to-noise.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eSMARCA4\u003c\/strong\u003e (also referred to as ATP dependent helicase SMARCA4, ATP-dependent helicase SMARCA4, BAF 190, BAF190, BAF190A, Brahma protein like 1, BRG1, BRG1 associated factor 190A, BRG1 protein, BRG1-associated factor 190A, BRM\/SWI2 related gene 1, Global transcription activator homologous sequence, global transcription activator snf2l4, Homeotic gene regulator, hSNF2b, Mitotic growth and transcription activator, MRD16, Nuclear protein GRB1, Protein brahma homolog 1, Protein BRG-1, Protein BRG1, RTPS2, SMARC A4, SMARCA4, SMCA4_HUMAN, SNF2, SNF2 beta, SNF2 like 4, SNF2-beta, SNF2B, SNF2L4, SNF2LB, Sucrose nonfermenting like 4, SWI\/SNF related matrix associated actin dependent regulator of chromatin subfamily A member 4, SWI\/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4, SWI\/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4, SWI2, Transcription activator BRG1) is a protein target studied in Human systems. Expression levels, subcellular localization, and post-translational modifications vary across cell types, tissues, and disease states — factors that influence antibody-based detection and experimental design. Consult UniProt, NCBI Gene, and primary literature for current annotation of SMARCA4 biology, including known isoforms, interactors, and disease-relevant expression patterns in neuroscience.\u003c\/p\u003e\n\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWB:\u003c\/strong\u003e Western blot confirms target molecular weight and distinguishes isoforms under denaturing conditions. Expected band size should align with reported MW.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e Sandwich or indirect ELISA can quantify soluble target in biological fluids or culture supernatants. Ensure samples fall within the linear detection range.\u003c\/li\u003e\n\n\u003c\/ul\u003e\n\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eIsotype controls:\u003c\/strong\u003e Use an isotype-matched (IgG from Rabbit) control at equivalent concentration to assess non-specific background.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCross-reactivity:\u003c\/strong\u003e Polyclonal preparations may cross-react with related proteins or isoforms. Orthogonal validation (siRNA, KO lysate, recombinant protein) is recommended to confirm signal specificity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMatrix effects:\u003c\/strong\u003e Sample matrix (serum, plasma, lysate, homogenate) can affect performance. Pilot dilution linearity and spike-recovery experiments are recommended for quantitative studies.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSpecies reactivity:\u003c\/strong\u003e Confirmed for Human, Mouse, Rat. Extrapolation to untested species requires empirical validation given potential epitope sequence divergence.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003cp\u003e\u003cem\u003eThis product is a polyclonal antibody purified from rabbit antiserum.\u003c\/em\u003e\u003c\/p\u003e\n\n\u003c!-- Sources (internal):\n- UniProt: https:\/\/www.uniprot.org\n- NCBI Gene: https:\/\/www.ncbi.nlm.nih.gov\/gene\n- Cusabio datasheet: https:\/\/www.cusabio.com\n- Human Protein Atlas: https:\/\/www.proteinatlas.org\n- PubMed: https:\/\/pubmed.ncbi.nlm.nih.gov\n--\u003e","brand":"CUSABIO TECHONOLOGY LLC","offers":[{"title":"50 ug","offer_id":53246253433197,"sku":"CSB-PA050103-50UG","price":119.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53246365761901,"sku":"CSB-PA050103-100UG","price":199.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/www.ebiohippo.com\/products\/smarca4-antibody-bha10541126","provider":"BioHippo","version":"1.0","type":"link"}