{"product_id":"smarcal1-antibody-harp-hepa-related-protein-bha17135242","title":"SMARCAL1 Antibody \/ HARP \/ HepA-related protein","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eSMARCAL1 Antibody \/ HARP \/ HepA-related protein is a anti-SMARCAL1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e SMARCAL1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eThe SMARCAL1 antibody targets SWI\/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1, a nuclear enzyme encoded by the SMARCAL1 gene. This ATP-dependent annealing helicase plays a vital role in DNA replication and repair, particularly in stabilizing stalled replication forks and preventing genome instability. The SMARCAL1 antibody provides a crucial research tool for investigating how cells respond to replication stress and maintain chromatin integrity under challenging conditions.\u003cbr\u003e\u003cbr\u003eSMARCAL1 is a member of the SNF2 family of chromatin remodelers, a large group of ATPases that modify DNA-protein interactions to facilitate replication and transcription. It is recruited to sites of replication fork arrest via interactions with replication protein A (RPA) and catalyzes branch migration to restart replication. This enzymatic process helps prevent double-strand breaks and genomic collapse. The SMARCAL1 antibody allows direct visualization of the protein in the nucleus, providing insight into its dynamics during S-phase and after genotoxic stress.\u003cbr\u003e\u003cbr\u003eMutations in SMARCAL1 are the primary cause of Schimke immuno-osseous dysplasia (SIOD), a rare autosomal recessive disorder marked by short stature, renal failure, and immune dysfunction. These phenotypes stem from impaired DNA repair and replication mechanisms, demonstrating the importance of SMARCAL1 in cellular viability and developmental stability. The SMARCAL1 antibody is therefore valuable not only in basic chromatin biology but also in studies of human disease and genomic maintenance.\u003cbr\u003e\u003cbr\u003eSMARCAL1's helicase activity is regulated by phosphorylation events mediated by ATR kinase and other components of the DNA damage response. These modifications influence its recruitment and activity at stalled forks. Researchers use the SMARCAL1 antibody in combination with phospho-specific markers to map these regulatory pathways. Western blotting enables detection of total protein levels, while immunofluorescence and confocal microscopy reveal its nuclear localization and accumulation at replication foci. Chromatin immunoprecipitation using the SMARCAL1 antibody further allows identification of genomic sites directly bound by the enzyme, shedding light on its mechanistic involvement in replication restart.\u003cbr\u003e\u003cbr\u003eIn broader biological contexts, SMARCAL1 contributes to genome stability, epigenetic maintenance, and transcriptional regulation through chromatin remodeling. Its deficiency can cause widespread transcriptional dysregulation and heightened sensitivity to replication stress. The SMARCAL1 antibody supplied by \u003cbr\u003e\u003cbr\u003eOngoing research using the SMARCAL1 antibody continues to clarify how this enzyme coordinates DNA replication and repair, how its dysfunction leads to disease, and how it might be therapeutically targeted to enhance genome stability. The combination of functional versatility and high-quality specificity makes the SMARCAL1 antibody a key reagent for understanding the complex interplay between chromatin structure, replication stress, and genomic maintenance.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=SMARCAL1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=SMARCAL1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=SMARCAL1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/SMARCAL1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=SMARCAL1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047284924781,"sku":"FY12339","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_50abb7b1-a2c0-4f18-9367-ef64207389e0.jpg?v=1782237006","url":"https:\/\/www.ebiohippo.com\/products\/smarcal1-antibody-harp-hepa-related-protein-bha17135242","provider":"BioHippo","version":"1.0","type":"link"}