{"product_id":"smpd3-antibody-sphingomyelin-phosphodiesterase-3-nsmase2-bha17136052","title":"SMPD3 Antibody \/ Sphingomyelin phosphodiesterase 3 \/ nSMase2","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eSMPD3 Antibody \/ Sphingomyelin phosphodiesterase 3 \/ nSMase2 is a anti-SMPD3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e SMPD3\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eSMPD3 antibody detects Sphingomyelin phosphodiesterase 3, a membrane-associated enzyme that hydrolyzes sphingomyelin to generate ceramide and phosphocholine. The UniProt recommended name is Sphingomyelin phosphodiesterase 3 (SMPD3). This enzyme, also known as neutral sphingomyelinase 2 (nSMase2), plays a key role in lipid metabolism, cell signaling, and exosome biogenesis.\u003cbr\u003e\u003cbr\u003eFunctionally, SMPD3 antibody identifies a 655-amino-acid enzyme localized to the plasma membrane and Golgi apparatus. SMPD3 catalyzes the production of ceramide, a lipid second messenger involved in apoptosis, differentiation, and inflammation. It regulates membrane curvature and vesicle budding, particularly in exosome secretion and cell stress responses.\u003cbr\u003e\u003cbr\u003eThe SMPD3 gene is located on chromosome 16p13.12 and is expressed in brain, liver, and skeletal tissue. SMPD3 activity influences cell growth, neuronal communication, and lipid raft organization. By controlling ceramide levels, SMPD3 links sphingolipid metabolism to cell fate decisions and inflammatory signaling.\u003cbr\u003e\u003cbr\u003ePathologically, dysregulation of SMPD3 contributes to metabolic, neurodegenerative, and cardiovascular diseases. Deficiency results in skeletal abnormalities (spondyloepimetaphyseal dysplasia) and altered lipid homeostasis. Overactivation can enhance ceramide-induced apoptosis or inflammatory cascades. Research using SMPD3 antibody supports studies in lipid signaling, neurobiology, and exosome biology.\u003cbr\u003e\u003cbr\u003eSMPD3 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect sphingomyelinases and lipid signaling enzymes. \u003cbr\u003e\u003cbr\u003eStructurally, Sphingomyelin phosphodiesterase 3 contains an N-terminal hydrophobic domain for membrane association and a C-terminal catalytic domain requiring Mg2+ for activity. This antibody enables analysis of SMPD3�s role in ceramide-mediated signal transduction and cellular stress responses.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=SMPD3 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=SMPD3 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=SMPD3 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/SMPD3 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=SMPD3+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047313269101,"sku":"FY13150","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_08355d01-56c1-4e7f-b352-07571b49b1ea.jpg?v=1782237092","url":"https:\/\/www.ebiohippo.com\/products\/smpd3-antibody-sphingomyelin-phosphodiesterase-3-nsmase2-bha17136052","provider":"BioHippo","version":"1.0","type":"link"}