{"product_id":"smtn-antibody-smoothelin-bha17136182","title":"SMTN Antibody \/ Smoothelin","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eSMTN Antibody \/ Smoothelin is a anti-SMTN Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm (Actin filaments), Nucleus.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e SMTN\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC, IF, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eSMTN antibody recognizes Smoothelin, a cytoskeletal protein encoded by the SMTN gene and specifically expressed in contractile smooth muscle cells. Smoothelin is a key component of the actin cytoskeleton that contributes to the mechanical stability and contractile function of differentiated smooth muscle. This protein localizes to stress fibers and dense bodies, where it interacts with actin filaments to support force generation and cell shape maintenance. SMTN exists in multiple isoforms, including smoothelin-A, primarily found in visceral smooth muscle, and smoothelin-B, which is more abundant in vascular smooth muscle. The expression of these isoforms reflects the functional specialization of different smooth muscle types in the gastrointestinal tract, bladder, and blood vessels.\u003cbr\u003e\u003cbr\u003eSmoothelin is tightly regulated during the phenotypic switch between contractile and synthetic smooth muscle states. During vascular injury or atherosclerotic changes, smooth muscle cells often downregulate SMTN expression as they transition to a proliferative phenotype, highlighting its role as a marker of mature, differentiated smooth muscle. Experimental models have shown that SMTN interacts with alpha-actinin, calponin, and other structural proteins to form an integrated cytoskeletal network essential for contractility. The gene is located on chromosome 22q12.3 and is transcriptionally regulated by serum response factor (SRF) and myocardin-related transcription factors, both central regulators of smooth muscle differentiation.\u003cbr\u003e\u003cbr\u003eImmunohistochemical analysis reveals strong SMTN staining in vascular media, intestinal muscularis, and detrusor muscle, whereas non-muscle tissues lack expression. Because of its restricted distribution, SMTN antibody is widely used in smooth muscle cell research, vascular biology, and tumor pathology to distinguish contractile smooth muscle cells from fibroblasts or myofibroblasts. Loss or reduction of SMTN has been associated with vascular remodeling, hypertension, and certain myopathies. The smoothelin protein is also of interest as a potential biomarker for monitoring smooth muscle differentiation in stem cell-derived tissue engineering systems.\u003cbr\u003e\u003cbr\u003eSMTN antibody from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=SMTN - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=SMTN - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=SMTN - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/SMTN - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=SMTN+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047320379757,"sku":"FY13280","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_b595730e-ac52-45f3-ba63-727c8e6ee5a0.jpg?v=1782237082","url":"https:\/\/www.ebiohippo.com\/products\/smtn-antibody-smoothelin-bha17136182","provider":"BioHippo","version":"1.0","type":"link"}