{"product_id":"smyd1-antibody-set-and-mynd-domain-containing-protein-1-bha17135572","title":"SMYD1 Antibody \/ SET and MYND domain-containing protein 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eSMYD1 Antibody \/ SET and MYND domain-containing protein 1 is a anti-SMYD1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e SMYD1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eSMYD1 antibody detects SET and MYND domain-containing protein 1, a muscle-specific histone methyltransferase that regulates gene expression during cardiac and skeletal muscle differentiation. SMYD1 modulates transcription by methylating histone H3 on lysine 4 and recruiting transcriptional cofactors to promoter regions of muscle-specific genes. The SMYD1 antibody is widely used in developmental biology and epigenetics research to study muscle gene regulation, histone modification, and cardiogenesis.\u003cbr\u003e\u003cbr\u003eSMYD1 is encoded by the SMYD1 gene located on human chromosome 2p11.2. The protein is approximately 490 amino acids long and contains an N-terminal SET domain responsible for methyltransferase activity and a C-terminal MYND zinc-finger domain that mediates protein-protein interactions. SMYD1 is predominantly expressed in heart and skeletal muscle tissues, where it coordinates transcriptional programs required for myofibril assembly and contractile function.\u003cbr\u003e\u003cbr\u003eThe SMYD1 antibody detects a 55 kilodalton band by western blot and shows nuclear and sarcoplasmic staining under immunofluorescence microscopy. SMYD1 acts as a transcriptional regulator of genes encoding structural and contractile proteins such as myosin heavy chains and troponins. It interacts with cofactors like HDACs and skNAC to coordinate repression and activation cycles during myogenesis.\u003cbr\u003e\u003cbr\u003eLoss of SMYD1 function in animal models results in defective cardiac morphogenesis, reduced sarcomere organization, and embryonic lethality, highlighting its critical developmental role. Dysregulation of SMYD1 expression has been linked to cardiomyopathy and muscular dystrophy, where abnormal histone methylation alters muscle gene networks. Beyond muscle, SMYD1 may influence differentiation of smooth muscle and endothelial lineages through epigenetic signaling.\u003cbr\u003e\u003cbr\u003eBecause SMYD1 couples chromatin modification to tissue-specific transcription, it represents a pivotal regulator of muscle differentiation and cardiac development.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=SMYD1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=SMYD1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=SMYD1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/SMYD1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=SMYD1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047296786797,"sku":"FY12669","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_f0ccc25b-86b9-498e-8ea4-27a03b08b985.jpg?v=1782237036","url":"https:\/\/www.ebiohippo.com\/products\/smyd1-antibody-set-and-mynd-domain-containing-protein-1-bha17135572","provider":"BioHippo","version":"1.0","type":"link"}