{"product_id":"snca-antibody-alpha-synuclein-bha17135739","title":"SNCA Antibody \/ Alpha Synuclein","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eSNCA Antibody \/ Alpha Synuclein is a anti-SNCA Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasmic, Nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e SNCA\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC, IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eSNCA antibody detects Alpha-synuclein, a small neuronal protein central to synaptic regulation, vesicle trafficking, and neurodegenerative disease pathology. Encoded by the SNCA gene on chromosome 4q22.1, Alpha-synuclein is a 140-amino acid presynaptic protein that modulates neurotransmitter release, synaptic vesicle recycling, and membrane curvature. It is highly expressed in the central nervous system, particularly in presynaptic terminals of dopaminergic neurons, where it helps maintain synaptic homeostasis. Despite its physiological role in neurotransmission, misfolded or aggregated forms of Alpha-synuclein are strongly implicated in Parkinson's disease and other synucleinopathies.\u003cbr\u003e\u003cbr\u003eAlpha-synuclein belongs to the synuclein family, which also includes Beta- and Gamma-synuclein. Structurally, it consists of three domains: an amphipathic N-terminal region that binds lipid membranes, a hydrophobic central non-amyloid-beta component (NAC) domain that promotes aggregation, and a highly acidic C-terminal region that interacts with synaptic vesicle components. These domains allow Alpha-synuclein to reversibly associate with synaptic vesicle membranes and regulate vesicle clustering, docking, and fusion events required for neurotransmitter release.\u003cbr\u003e\u003cbr\u003eThe SNCA antibody is widely used in neuroscience, neurodegenerative disease, and cellular biology research to study protein aggregation, synaptic function, and neuronal homeostasis. Western blot analysis identifies a 17 kilodalton band corresponding to Alpha-synuclein, while immunohistochemistry and immunofluorescence reveal diffuse cytoplasmic and presynaptic punctate staining in neurons. In pathological tissue, the antibody recognizes characteristic Lewy bodies and Lewy neurites formed by Alpha-synuclein aggregates, providing a valuable diagnostic marker for Parkinson's disease and dementia with Lewy bodies.\u003cbr\u003e\u003cbr\u003ePhysiologically, Alpha-synuclein interacts with SNARE complex proteins such as VAMP2 and synaptobrevin to facilitate vesicle fusion, while regulating dopamine release and synaptic plasticity. However, environmental stress, oxidative damage, or genetic mutations (e.g., A53T, A30P, E46K) can cause Alpha-synuclein misfolding and fibril formation. These aggregates trigger mitochondrial dysfunction, ER stress, and neuronal death through toxic oligomer formation. The SNCA antibody is an essential tool for studying these mechanisms and evaluating therapeutic interventions aimed at reducing aggregation or promoting clearance via autophagy and proteasomal degradation.\u003cbr\u003e\u003cbr\u003eAlpha-synuclein also plays a role in non-neuronal systems, including erythrocytes and the enteric nervous system, where it may influence vesicular trafficking and lipid metabolism. Dysregulation of SNCA transcription and post-translational modifications such as phosphorylation (Ser129), nitration, or ubiquitination modulate its aggregation behavior and toxicity. The SNCA antibody supports high-resolution mapping of these biochemical changes, helping researchers understand disease progression and identify novel treatment strategies.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=SNCA - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=SNCA - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=SNCA - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/SNCA - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=SNCA+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047303438701,"sku":"FY12837","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_3bcd9bf2-7259-4200-aa12-7e2a99d081c4.jpg?v=1782237052","url":"https:\/\/www.ebiohippo.com\/products\/snca-antibody-alpha-synuclein-bha17135739","provider":"BioHippo","version":"1.0","type":"link"}