{"product_id":"snx16-antibody-sorting-nexin-16-bha17135404","title":"SNX16 Antibody \/ Sorting nexin-16","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eSNX16 Antibody \/ Sorting nexin-16 is a anti-SNX16 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e SNX16\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC\/IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eSNX16 antibody detects Sorting nexin-16, a phosphoinositide-binding protein that regulates endosomal trafficking, membrane remodeling, and receptor recycling. SNX16 belongs to the sorting nexin family, which is characterized by the presence of a phox homology (PX) domain that interacts with phosphatidylinositol 3-phosphate-enriched membranes. The SNX16 antibody is widely used in cell biology and neurobiology research to investigate endosome organization and cargo transport.\u003cbr\u003e\u003cbr\u003eSNX16 is encoded by the SNX16 gene located on human chromosome 8p21.3. The protein is approximately 62 kilodaltons and localizes primarily to early endosomes. It participates in the formation of tubular endosomal structures that mediate receptor sorting between degradation and recycling pathways. SNX16 functions independently of the retromer complex and instead promotes membrane tubulation through its coiled-coil domain. It regulates trafficking of receptors such as EGFR and TrkB, influencing growth factor signaling and neuronal development.\u003cbr\u003e\u003cbr\u003eThe SNX16 antibody typically identifies a 40-50 kilodalton protein by western blot and demonstrates punctate cytoplasmic staining characteristic of endosomal localization. Functional studies show that depletion of SNX16 disrupts endosomal tubulation, alters cargo recycling, and can impair signaling pathways dependent on receptor localization. Overexpression of SNX16 enhances endosomal membrane curvature and promotes receptor clustering, demonstrating its mechanochemical role in shaping endosomes.\u003cbr\u003e\u003cbr\u003eIn neurons, SNX16 contributes to dendritic spine maintenance and receptor turnover, impacting synaptic plasticity. It may also participate in autophagy regulation through endosome-lysosome fusion. Aberrant SNX16 expression has been linked to neurodegenerative disorders and cancer, where altered trafficking pathways contribute to signaling dysregulation.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=SNX16 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=SNX16 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=SNX16 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/SNX16 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=SNX16+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047290790253,"sku":"FY12501","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_64d3aa20-2b41-485c-a081-e170589fe530.jpg?v=1782237018","url":"https:\/\/www.ebiohippo.com\/products\/snx16-antibody-sorting-nexin-16-bha17135404","provider":"BioHippo","version":"1.0","type":"link"}