{"product_id":"snx2-antibody-sorting-nexin-2-bha17135900","title":"SNX2 Antibody \/ Sorting nexin 2","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eSNX2 Antibody \/ Sorting nexin 2 is a anti-SNX2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e SNX2\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eSNX2 antibody detects Sorting nexin-2, a membrane-associated protein involved in endosomal sorting, receptor trafficking, and retrograde transport from endosomes to the trans-Golgi network. The UniProt recommended name is Sorting nexin-2 (SNX2). This protein belongs to the sorting nexin family characterized by a phosphoinositide-binding PX (phox homology) domain that mediates association with endosomal membranes rich in phosphatidylinositol 3-phosphate.\u003cbr\u003e\u003cbr\u003eFunctionally, SNX2 antibody identifies a 521-amino-acid cytoplasmic protein that localizes to early and recycling endosomes. SNX2 functions as a key component of the retromer complex, which retrieves cargo proteins from endosomes for recycling to the Golgi apparatus or plasma membrane. It works in concert with other retromer subunits such as VPS26, VPS29, and VPS35 to ensure proper sorting and trafficking of membrane receptors including mannose-6-phosphate receptor and Wntless.\u003cbr\u003e\u003cbr\u003eThe SNX2 gene is located on chromosome 5q23.3 and encodes a protein ubiquitously expressed in eukaryotic cells. SNX2 contains a PX domain that binds phosphoinositides and a BAR (Bin\/Amphiphysin\/Rvs) domain responsible for membrane curvature sensing and tubulation. Together, these domains enable SNX2 to shape endosomal membranes and facilitate cargo vesicle formation. It also interacts with clathrin and dynamin to coordinate endocytic vesicle budding and recycling.\u003cbr\u003e\u003cbr\u003eIn cellular signaling, SNX2 regulates the trafficking of growth factor receptors such as EGFR, modulating receptor downregulation and signal duration. It contributes to Wnt pathway regulation by recycling Wntless, essential for ligand secretion. Disruption of SNX2 impairs endosome-to-Golgi transport, leading to accumulation of misrouted cargo and altered cell signaling. SNX2 has also been implicated in pathogen entry and immune receptor trafficking.\u003cbr\u003e\u003cbr\u003eSNX2 antibody is widely used in membrane trafficking, signal transduction, and endocytosis research. It is suitable for immunoblotting, immunofluorescence, and subcellular localization studies to visualize endosomal compartments. In neurobiology, SNX2 detection supports studies of synaptic vesicle recycling and neurodegenerative disorders linked to retromer dysfunction. Its expression is also examined in cancer biology, where altered trafficking contributes to aberrant receptor signaling and invasion.\u003cbr\u003e\u003cbr\u003eStructurally, SNX2 functions as a dimer that induces membrane tubulation and curvature through its BAR domain, while its PX domain anchors the complex to phosphoinositide-rich membranes.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=SNX2 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=SNX2 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=SNX2 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/SNX2 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=SNX2+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047308976493,"sku":"FY12998","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_3b9807b1-6009-4688-9848-413cd4e446f9.jpg?v=1782237063","url":"https:\/\/www.ebiohippo.com\/products\/snx2-antibody-sorting-nexin-2-bha17135900","provider":"BioHippo","version":"1.0","type":"link"}