{"product_id":"son-antibody-protein-son-bha17135883","title":"SON Antibody \/ Protein SON","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eSON Antibody \/ Protein SON is a anti-SON Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse. Reported localization: Nuclear speckles.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e SON\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, IF, ICC\/IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eSON antibody detects Protein SON, a large nuclear RNA-binding protein involved in pre-mRNA splicing, transcriptional regulation, and genome organization. The UniProt recommended name is Protein SON (SON), a multifunctional factor that facilitates RNA processing and gene expression fidelity. SON plays critical roles in maintaining nuclear speckle organization, coordinating spliceosome function, and ensuring accurate mRNA maturation.\u003cbr\u003e\u003cbr\u003eFunctionally, SON antibody identifies a 2,426-amino-acid nuclear protein characterized by multiple low-complexity regions, a G-patch domain, and RNA recognition motifs. SON interacts with splicing factors, including SRSF2, PRPF8, and U2AF65, promoting efficient intron removal and exon definition. It acts as a scaffold within nuclear speckles, organizing splicing regulators and transcription machinery. SON also participates in the transcriptional regulation of genes governing cell cycle progression, differentiation, and chromatin stability.\u003cbr\u003e\u003cbr\u003eThe SON gene is located on chromosome 21q22.11 and is highly conserved across vertebrates. It is ubiquitously expressed but enriched in proliferating cells, reflecting its role in transcription-coupled RNA processing. Depletion of SON results in global splicing defects, preferentially affecting long genes with weak splice sites and GC-rich regions. These defects lead to downregulation of critical regulators of DNA replication, chromosome segregation, and pluripotency.\u003cbr\u003e\u003cbr\u003eMutations or haploinsufficiency of SON cause ZTTK syndrome (Zhu-Tokita-Takenouchi-Kim syndrome), a developmental disorder characterized by intellectual disability, growth retardation, and brain malformations. SON also influences genome organization by tethering active chromatin domains near nuclear speckles, thereby enhancing transcriptional efficiency. In cancer, overexpression of SON supports rapid proliferation and survival by stabilizing mRNAs of oncogenic pathways, including MYC and WNT signaling components.\u003cbr\u003e\u003cbr\u003eSON antibody is widely used in molecular biology and nuclear organization research. It is suitable for immunoblotting, immunofluorescence, and RNA immunoprecipitation to examine SON's role in splicing and chromatin architecture. SON serves as a nuclear speckle marker in microscopy, distinguishing transcriptionally active nuclear domains. In developmental biology, SON detection aids in characterizing splicing fidelity and gene expression networks critical for tissue differentiation.\u003cbr\u003e\u003cbr\u003eStructurally, SON contains numerous serine\/arginine-rich and glycine-rich motifs that mediate protein and RNA interactions. Phosphorylation regulates its association with spliceosomal complexes and nuclear bodies.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=SON - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=SON - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=SON - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/SON - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=SON+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047307993453,"sku":"FY12981","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_01b5449a-3db2-408b-a51c-fa9f4370faec.jpg?v=1782237067","url":"https:\/\/www.ebiohippo.com\/products\/son-antibody-protein-son-bha17135883","provider":"BioHippo","version":"1.0","type":"link"}