{"product_id":"sos1-antibody-son-of-sevenless-1-bha17135486","title":"SOS1 Antibody \/ Son of sevenless 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eSOS1 Antibody \/ Son of sevenless 1 is a anti-SOS1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm, cell membrane.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e SOS1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eSOS1 antibody detects Son of sevenless homolog 1, a guanine nucleotide exchange factor (GEF) that activates Ras and Rac GTPases, serving as a critical link between receptor tyrosine kinases and downstream signaling cascades. SOS1 catalyzes the exchange of GDP for GTP on Ras, thereby triggering MAPK and PI3K pathway activation. The SOS1 antibody is widely used in cell signaling, oncology, and developmental biology to study Ras-mediated signal transduction and growth control.\u003cbr\u003e\u003cbr\u003eSOS1 is encoded by the SOS1 gene on human chromosome 2p22.1. The protein is approximately 1333 amino acids long and consists of several domains: an N-terminal histone-like fold, a Dbl homology (DH) domain, a pleckstrin homology (PH) domain, and a C-terminal catalytic region responsible for Ras activation. SOS1 is recruited to the plasma membrane through interaction with adaptor proteins such as GRB2 following growth factor stimulation.\u003cbr\u003e\u003cbr\u003eThe SOS1 antibody detects a 155 kilodalton protein in western blot assays and shows cytoplasmic and membrane localization by immunofluorescence. Upon receptor activation, SOS1 facilitates Ras activation at the plasma membrane, leading to phosphorylation of ERK and other downstream targets. Through its DH-PH domains, SOS1 also activates Rac, linking actin cytoskeleton remodeling to growth factor signaling.\u003cbr\u003e\u003cbr\u003eSOS1 plays essential roles in development, cell proliferation, and differentiation. Germline mutations in SOS1 cause Noonan syndrome, a developmental disorder characterized by short stature and congenital heart defects. In cancer, overexpression or dysregulation of SOS1 contributes to aberrant Ras activation and uncontrolled growth signaling. Its activity is finely tuned by feedback loops involving phosphorylation, ubiquitination, and membrane recruitment dynamics.\u003cbr\u003e\u003cbr\u003eBecause of its central position in the Ras signaling cascade, SOS1 is a valuable target for understanding receptor-driven proliferation and oncogenic transformation.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=SOS1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=SOS1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=SOS1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/SOS1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=SOS1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047293149549,"sku":"FY12583","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_111c91bd-4b3e-4a66-bd48-cdefa6badaed.jpg?v=1782237021","url":"https:\/\/www.ebiohippo.com\/products\/sos1-antibody-son-of-sevenless-1-bha17135486","provider":"BioHippo","version":"1.0","type":"link"}