{"product_id":"spa17-antibody-sperm-autoantigenic-protein-17-bha17136193","title":"SPA17 Antibody \/ Sperm autoantigenic protein 17","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eSPA17 Antibody \/ Sperm autoantigenic protein 17 is a anti-SPA17 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB) with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm (Golgi), cell memebrane.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e SPA17\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e ELISA, IF, IHC, WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eSPA17 antibody recognizes Sperm autoantigenic protein 17, a testis-enriched protein encoded by the SPA17 gene that is involved in sperm motility, fertilization, and cell adhesion. SPA17 is expressed primarily in the acrosomal region of mature spermatozoa and in ciliated epithelial tissues, where it contributes to cell-cell interaction and immune recognition processes. The protein contains an N-terminal sperm-coating antigen domain and a C-terminal region homologous to the heat shock protein family, suggesting a chaperone-like role in maintaining sperm surface protein stability.\u003cbr\u003e\u003cbr\u003eSPA17 was initially identified as an autoantigen in patients with infertility and autoimmune disorders, reflecting its immunogenic nature. It is also classified as a cancer-testis antigen due to its restricted normal expression in testicular tissue and aberrant expression in various malignancies, including ovarian, prostate, and lung cancers. This re-expression in tumors has made SPA17 a target of interest for cancer immunotherapy and tumor biomarker research. Functionally, SPA17 promotes sperm-egg interaction by binding to carbohydrate residues on the zona pellucida and may participate in intracellular signaling during acrosomal exocytosis.\u003cbr\u003e\u003cbr\u003eThe SPA17 gene is located on chromosome 11q24.2 and encodes a 151-amino acid protein that forms homodimers. The protein associates with the outer acrosomal membrane and flagellar structures, supporting motility and fertilization capacity. Beyond reproduction, SPA17 has been detected in immune and neural tissues, where it may modulate cAMP-mediated signaling pathways. Studies have shown SPA17 interaction with protein kinase A (PKA) regulatory subunits, linking it to cellular adhesion and signal transduction.\u003cbr\u003e\u003cbr\u003eImmunohistochemical analysis using SPA17 antibody reveals strong staining in testis, epididymis, and certain tumor tissues. The antibody is widely used to investigate sperm biology, fertility disorders, and tumor immunogenicity. SPA17 antibody from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=SPA17 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=SPA17 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=SPA17 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/SPA17 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=SPA17+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047321493869,"sku":"FY13291","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_f6a93df6-f570-431d-ada8-a9ad65485ebf.jpg?v=1782237097","url":"https:\/\/www.ebiohippo.com\/products\/spa17-antibody-sperm-autoantigenic-protein-17-bha17136193","provider":"BioHippo","version":"1.0","type":"link"}