{"product_id":"spata21-antibody-spermatogenesis-associated-protein-21-bha17135532","title":"SPATA21 Antibody \/ Spermatogenesis-associated protein 21","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eSPATA21 Antibody \/ Spermatogenesis-associated protein 21 is a anti-SPATA21 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e SPATA21\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eSPATA21 antibody detects Spermatogenesis-associated protein 21, a testis-enriched protein implicated in sperm maturation, motility, and flagellar assembly. SPATA21 plays a critical role in the later stages of spermatogenesis and is essential for normal sperm structure and fertilization competence. The SPATA21 antibody is widely used in reproductive biology and developmental studies to explore germ cell differentiation and fertility regulation.\u003cbr\u003e\u003cbr\u003eSPATA21 is encoded by the SPATA21 gene located on human chromosome 1p36.33. The protein is approximately 678 amino acids in length and localizes to the cytoplasm and flagellum of developing spermatids. It interacts with axonemal and mitochondrial sheath proteins, supporting assembly of sperm tail components and providing energy efficiency during motility.\u003cbr\u003e\u003cbr\u003eThe SPATA21 antibody detects an 80 kilodalton band by western blot and exhibits strong staining in testicular germ cells under immunofluorescence. Functional studies suggest SPATA21 participates in mitochondrial tethering to the flagellum and regulation of axoneme structure, contributing to sperm morphology and motility. Defects in SPATA21 expression lead to abnormal sperm development, reduced motility, and male infertility.\u003cbr\u003e\u003cbr\u003eSPATA21 may also play roles in germ cell apoptosis and stress responses during spermatogenesis. It interacts with chaperones and cytoskeletal proteins to stabilize protein complexes under oxidative stress. Comparative studies show high conservation across species, emphasizing its evolutionary significance in reproductive biology.\u003cbr\u003e\u003cbr\u003eBecause of its specialized role in sperm development, SPATA21 serves as a valuable marker for testicular function and reproductive competence.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=SPATA21 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=SPATA21 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=SPATA21 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/SPATA21 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=SPATA21+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047294525805,"sku":"FY12629","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_dd209a8a-487c-4b69-9489-f36e4f0460df.jpg?v=1782237021","url":"https:\/\/www.ebiohippo.com\/products\/spata21-antibody-spermatogenesis-associated-protein-21-bha17135532","provider":"BioHippo","version":"1.0","type":"link"}