{"product_id":"spata6-antibody-spermatogenesis-associated-protein-6-bha17135338","title":"SPATA6 Antibody \/ Spermatogenesis-associated protein 6","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eSPATA6 Antibody \/ Spermatogenesis-associated protein 6 is a anti-SPATA6 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Golgi apparatus, Vesicles.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e SPATA6\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eThe SPATA6 antibody targets Spermatogenesis-associated protein 6, a membrane-anchored protein encoded by the SPATA6 gene. Spermatogenesis-associated protein 6 plays a crucial role in sperm tail formation, flagellar assembly, and intercellular junction organization during spermatid maturation. It localizes primarily to the manchette and head-tail coupling apparatus, structures that facilitate shaping and attachment of the developing sperm tail. The SPATA6 antibody allows researchers to study sperm morphogenesis, ciliary structure, and reproductive cell differentiation. \u003cbr\u003e\u003cbr\u003eSpermatogenesis-associated protein 6 contributes to the structural integrity of the connecting piece between the nucleus and flagellum, ensuring proper transmission of motility forces. The SPATA6 antibody supports visualization of this protein during spermiogenesis, revealing dynamic redistribution patterns correlated with microtubule organization. Loss of SPATA6 disrupts flagellar biogenesis and causes male infertility in model organisms, emphasizing its essential role in germ-cell architecture. \u003cbr\u003e\u003cbr\u003eBeyond testicular expression, Spermatogenesis-associated protein 6 is detected in ciliated epithelial cells, suggesting broader participation in cilium assembly and anchoring. The SPATA6 antibody facilitates these studies and aids in understanding conserved mechanisms that govern axoneme formation and basal-body docking. Its structural role links it to cytoskeletal regulators and motor proteins such as dyneins and kinesins. \u003cbr\u003e\u003cbr\u003eMutations in the SPATA6 gene have been associated with asthenozoospermia, characterized by reduced sperm motility. The SPATA6 antibody enables quantification of protein expression in sperm samples and cell lines, providing valuable diagnostic and mechanistic insights. Additionally, SPATA6 participates in the assembly of intercellular junctions during germ-cell development, influencing Sertoli�spermatid adhesion and polarity. \u003cbr\u003e\u003cbr\u003eThe SPATA6 antibody performs effectively in western blotting, immunohistochemistry, and immunofluorescence, typically showing localization to sperm tail and manchette regions.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=SPATA6 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=SPATA6 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=SPATA6 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/SPATA6 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=SPATA6+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047288791405,"sku":"FY12435","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_744e173c-bc5f-4f10-939d-fadb1df338e3.jpg?v=1782237008","url":"https:\/\/www.ebiohippo.com\/products\/spata6-antibody-spermatogenesis-associated-protein-6-bha17135338","provider":"BioHippo","version":"1.0","type":"link"}