{"product_id":"spidr-antibody-scaffold-protein-involved-in-dna-repair-bha17135686","title":"SPIDR Antibody \/ Scaffold protein involved in DNA repair","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eSPIDR Antibody \/ Scaffold protein involved in DNA repair is a anti-SPIDR Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Western blot (WB), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat. Reported localization: Nucleus.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e SPIDR\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e ELISA, IF, ICC, WB, FACS\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eSPIDR antibody detects Scaffold protein involved in DNA repair (also known as KIAA0146), a nuclear adaptor protein that coordinates homologous recombination and DNA double-strand break repair. Encoded by the SPIDR gene on chromosome 8q24.3, this protein functions as a scaffold linking key repair enzymes including RAD51, BRCA2, and RPA to the DNA damage response machinery. SPIDR facilitates the assembly of repair complexes at damaged DNA sites and promotes strand invasion and exchange during homologous recombination.\u003cbr\u003e\u003cbr\u003eSPIDR contains multiple interaction domains, including coiled-coil regions and binding motifs that mediate association with RAD51 paralogs and BRCA2. Following DNA damage, SPIDR localizes to nuclear foci corresponding to double-strand break sites, where it promotes RAD51 filament formation and repair fidelity. By stabilizing repair intermediates, SPIDR ensures proper resolution of recombination events and maintenance of genomic stability. Depletion of SPIDR impairs DNA repair efficiency and increases chromosomal aberrations, underscoring its importance in genome maintenance.\u003cbr\u003e\u003cbr\u003eThe SPIDR antibody is used in DNA repair, cancer, and cell cycle research to analyze homologous recombination pathways and genomic stability. Western blot analysis identifies a 90 kilodalton band corresponding to SPIDR, while immunofluorescence shows punctate nuclear foci following genotoxic stress. This antibody enables researchers to track DNA repair factor recruitment and assess homologous recombination efficiency.\u003cbr\u003e\u003cbr\u003eDefects in SPIDR expression or localization are associated with hypersensitivity to DNA-damaging agents and increased genomic instability, contributing to cancer susceptibility. SPIDR also cooperates with checkpoint proteins such as ATR and CHK1 to coordinate DNA repair with replication stress responses. The SPIDR antibody supports these investigations by providing specific detection in western blotting, flow cytometry, and immunofluorescence applications.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=SPIDR - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=SPIDR - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=SPIDR - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/SPIDR - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=SPIDR+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047299703149,"sku":"FY12783","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_175bf6de-10a8-48e7-8c93-a68b7e231732.jpg?v=1782237055","url":"https:\/\/www.ebiohippo.com\/products\/spidr-antibody-scaffold-protein-involved-in-dna-repair-bha17135686","provider":"BioHippo","version":"1.0","type":"link"}