{"product_id":"spin-column-membrane-protein-folding-screen-kit-bht16300167","title":"Spin-Column Membrane Protein Folding Screen Kit","description":"\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}.bhp-desc ol,.bhp-desc ul{margin:0 0 12px 20px}\u003c\/style\u003e\u003cdiv class=\"bhp-desc\"\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eMembrane Protein Folding\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eThe Spin-Column Membrane Protein Folding Screen Kit is designed for folding membrane proteins from inclusion bodies using spin columns. Its 20 prepacked spin columns represent 20 folding conditions with different detergents and lipids that form micelles or bicelles; these environments help fold receptors, ion channels and other membrane proteins.\u003c\/p\u003e\n\u003cp\u003eThe folded samples from the screen are checked by SDS-PAGE and activity tests, and the best condition (column number) is then used for preparative folding with the matching Preparative Membrane Protein Folding Column Set.\u003c\/p\u003e\n\u003ch2\u003eProduct Description\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctr\u003e\n\u003cth\u003eScreen kit (20 different conditions, one column each, #1–20) – MFC01-20\u003c\/th\u003e\n\u003ctd\u003e20 prepacked spin columns (#1–20), 160 µl Reagent A, 160 µl Reagent B and 1.1 ml Reagent C\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/table\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eFormat\u003c\/th\u003e\n\u003ctd\u003e20 prepacked spin columns (#1–20)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eReagents\u003c\/th\u003e\n\u003ctd\u003e160 µl Reagent A, 160 µl Reagent B, 1.1 ml Reagent C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eColumns #1–10\u003c\/th\u003e\n\u003ctd\u003eUsed with Reagent A\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eColumns #11–20\u003c\/th\u003e\n\u003ctd\u003eUsed with Reagent B\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStarting material\u003c\/th\u003e\n\u003ctd\u003eSolubilized inclusion bodies, about 2–5 mg\/ml protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eCatalog #\u003c\/th\u003e\n\u003ctd\u003eMFC01-20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003ePlease contact us to confirm storage conditions.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003eShipped frozen.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eProtocol Outline\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003ePrepare Loading Sample A (130 µl solubilized inclusion bodies + 130 µl Reagent A) for columns #1–10 and Loading Sample B (with Reagent B) for columns #11–20.\u003c\/li\u003e\n\u003cli\u003eSpin the columns at 3200 rpm for 30 s, remove the bottom tips and caps, spin at 1400 rpm for 2 min and move each column to a clean tube.\u003c\/li\u003e\n\u003cli\u003eLoad 25 µl of the matching loading sample onto each column and spin at 3200 rpm for 4 min.\u003c\/li\u003e\n\u003cli\u003eIncubate the eluates at 4°C for 2 h, add 50 µl Reagent C, incubate at 4°C overnight, spin at 14,000 rpm for 5 min and analyze the supernatant by SDS-PAGE and activity tests.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003eThe ProFoldin protocol “Spin-column Membrane Protein Folding Screen” is linked under Documents.\u003c\/p\u003e\n\u003ch2\u003eNotes\u003c\/h2\u003e\n\u003cul\u003e\u003cli\u003eCatalog # MFC01-20 is this screen kit: 20 spin columns, each a different folding condition (#1–20). Catalog #s MFC01 to MFC20 and MFC01S to MFC20S are the preparative column sets (Preparative Membrane Protein Folding Column Set), each with several columns of one selected condition.\u003c\/li\u003e\u003c\/ul\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003eFor Research Use Only (RUO). Not for use in diagnostic or therapeutic procedures. Follow standard laboratory safety practices.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"ProFoldin","offers":[{"title":"Screen kit (20 different conditions, one column each, #1–20)","offer_id":67828319453549,"sku":"MFC01-20","price":346.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/spin_column-membrane.gif?v=1793129658","url":"https:\/\/www.ebiohippo.com\/products\/spin-column-membrane-protein-folding-screen-kit-bht16300167","provider":"BioHippo","version":"1.0","type":"link"}