{"product_id":"spin-column-protein-folding-screen-kit-bht16300169","title":"Spin-Column Protein Folding Screen Kit","description":"\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}.bhp-desc ol,.bhp-desc ul{margin:0 0 12px 20px}\u003c\/style\u003e\u003cdiv class=\"bhp-desc\"\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eProtein Folding\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eThe Spin-Column Protein Folding Screen Kit is designed to produce active proteins from guanidine hydrochloride- or urea-solubilized inclusion bodies, or from protein aggregates formed during expression, purification or storage. Different proteins fold under different conditions, so the kit provides 10 spin columns representing 10 diverse folding conditions, including conditions that allow disulfide bond formation and reducing conditions.\u003c\/p\u003e\n\u003cp\u003eThe folded samples are checked by SDS-PAGE and activity tests, and the best condition (column number) is then used for preparative folding with the matching Preparative Protein Folding Column Set.\u003c\/p\u003e\n\u003ch2\u003eProduct Description\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctr\u003e\n\u003cth\u003eScreen kit (10 different conditions, one column each, #1–10) – SFC01-10\u003c\/th\u003e\n\u003ctd\u003e10 spin columns (#1–10), 160 µl Solution A and 600 µl Solution B\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/table\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eFormat\u003c\/th\u003e\n\u003ctd\u003e10 spin columns (#1–10)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eReagents\u003c\/th\u003e\n\u003ctd\u003e160 µl Solution A, 600 µl Solution B\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStarting material\u003c\/th\u003e\n\u003ctd\u003eGuanidine hydrochloride- or urea-solubilized inclusion bodies\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eCatalog #\u003c\/th\u003e\n\u003ctd\u003eSFC01-10\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003ePlease contact us to confirm storage conditions.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003eShipped cold (not frozen).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eProtocol Outline\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eSolubilize inclusion bodies in 20 mM Tris-HCl, pH 8.0, 6 M guanidine hydrochloride (or 8 M urea), 10 mM DTT, and clear by centrifugation.\u003c\/li\u003e\n\u003cli\u003eKeep the columns and reagents at 2–8°C. Mix 130 µl of solubilized inclusion bodies with 130 µl of Solution A and incubate for 5 min.\u003c\/li\u003e\n\u003cli\u003eSpin the columns at 3200 rpm for 1 min, remove the bottom tips and caps, spin at 1400 rpm for 2 min and move each column to a clean tube.\u003c\/li\u003e\n\u003cli\u003eLoad 25 µl of the loading sample onto each column and spin at 3200 rpm for 4 min; incubate the eluates at 4°C for 2–4 h, add 50 µl Solution B, incubate at 4°C for 2 h to overnight, spin at 14,000 rpm for 5 min and analyze the supernatant by SDS-PAGE and activity tests.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003eThe ProFoldin protocol “Spin-Column Protein Folding Screen” is linked under Documents.\u003c\/p\u003e\n\u003ch2\u003eNotes\u003c\/h2\u003e\n\u003cul\u003e\u003cli\u003eCatalog # SFC01-10 is this screen kit: 10 spin columns, each a different folding condition (#1–10). Catalog #s SFC01 to SFC10 (without -10) are the small-scale preparative sets of the Preparative Protein Folding Column Set, each with 10 identical columns of one selected condition.\u003c\/li\u003e\u003c\/ul\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003eFor Research Use Only (RUO). Not for use in diagnostic or therapeutic procedures. Follow standard laboratory safety practices.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"ProFoldin","offers":[{"title":"Screen kit (10 different conditions, one column each, #1–10)","offer_id":67828319420781,"sku":"SFC01-10","price":240.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/www.ebiohippo.com\/products\/spin-column-protein-folding-screen-kit-bht16300169","provider":"BioHippo","version":"1.0","type":"link"}