{"product_id":"spink4-antibody-serine-peptidase-inhibitor-kazal-type-4-bha17136088","title":"SPINK4 Antibody \/ Serine peptidase inhibitor Kazal-type 4","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eSPINK4 Antibody \/ Serine peptidase inhibitor Kazal-type 4 is a anti-SPINK4 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Immunohistochemistry (IHC) with listed reactivity in Human. Reported localization: Golgi, Nucleus.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e SPINK4\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e IHC\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eSPINK4 antibody detects Serine peptidase inhibitor Kazal-type 4, a secreted protease inhibitor that regulates intestinal mucosal protection and epithelial homeostasis. The UniProt recommended name is Serine peptidase inhibitor Kazal-type 4 (SPINK4). This small protein is part of the Kazal-type serine protease inhibitor family and functions as a local regulator of proteolytic activity in gastrointestinal tissues.\u003cbr\u003e\u003cbr\u003eFunctionally, SPINK4 antibody identifies a 86-amino-acid protein predominantly expressed in goblet cells of the small and large intestine. SPINK4 binds and inhibits trypsin-like proteases, preventing excessive proteolysis in the mucosal environment. It contributes to epithelial integrity and immune defense by maintaining the protective mucus barrier.\u003cbr\u003e\u003cbr\u003eThe SPINK4 gene is located on chromosome 9p13.3 and is expressed specifically in intestinal and colonic mucosa. Its expression is upregulated during inflammation and epithelial injury, reflecting its protective and regulatory role in the gut.\u003cbr\u003e\u003cbr\u003ePathologically, elevated SPINK4 levels have been reported in colorectal cancer, inflammatory bowel disease, and gastrointestinal infections. It is a potential biomarker for epithelial stress and differentiation states in intestinal disorders. Research using SPINK4 antibody supports studies in mucosal immunology, epithelial biology, and cancer biomarker discovery.\u003cbr\u003e\u003cbr\u003eSPINK4 antibody is validated for western blotting, ELISA, and immunohistochemistry to detect secreted protease inhibitors. \u003cbr\u003e\u003cbr\u003eStructurally, Serine peptidase inhibitor Kazal-type 4 consists of a single Kazal inhibitory domain stabilized by disulfide bonds, which confers high affinity for target proteases. This antibody enables investigation of SPINK4's function in mucosal protection and intestinal homeostasis.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=SPINK4 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=SPINK4 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=SPINK4 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/SPINK4 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=SPINK4+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047314743661,"sku":"FY13186","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_dd59ad70-962c-418d-be45-166c9917d950.jpg?v=1782237100","url":"https:\/\/www.ebiohippo.com\/products\/spink4-antibody-serine-peptidase-inhibitor-kazal-type-4-bha17136088","provider":"BioHippo","version":"1.0","type":"link"}