{"product_id":"spink5-antibody-serine-protease-inhibitor-kazal-type-5-bha17135297","title":"Spink5 Antibody \/ Serine protease inhibitor Kazal-type 5","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eSpink5 Antibody \/ Serine protease inhibitor Kazal-type 5 is a anti-SPINK5 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e SPINK5\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eThe SPINK5 antibody targets Serine protease inhibitor Kazal-type 5, a secreted protein encoded by the SPINK5 gene that functions as a multi-domain protease inhibitor. Serine protease inhibitor Kazal-type 5 plays a vital role in maintaining epithelial barrier integrity by regulating protease activity within the skin and mucosal surfaces. The protein contains multiple Kazal-type domains that inhibit trypsin-like and chymotrypsin-like proteases, preventing excessive proteolysis of structural and immune components. The SPINK5 antibody enables detection of this essential regulatory protein in epithelial tissues and supports research into skin biology and inflammatory diseases.\u003cbr\u003e\u003cbr\u003eSerine protease inhibitor Kazal-type 5 is most highly expressed in the epidermis, particularly in the granular and cornified layers, where it contributes to desquamation and barrier formation. It inhibits kallikreins such as KLK5, KLK7, and KLK14, which are involved in corneodesmosome degradation. The SPINK5 antibody allows investigation of this balance between protease activation and inhibition, providing insight into how SPINK5 ensures skin homeostasis and prevents barrier dysfunction. Mutations or loss of expression lead to unrestrained proteolytic activity, resulting in the autosomal recessive disorder Netherton syndrome.\u003cbr\u003e\u003cbr\u003eNetherton syndrome is characterized by ichthyosiform dermatitis, hair shaft abnormalities, and elevated IgE levels due to defective SPINK5-mediated protease control. The SPINK5 antibody is an important diagnostic and research tool for studying the molecular mechanisms underlying this condition. It enables detection of reduced or absent protein expression in patient tissues, confirming pathogenic variants. Experimental models show that loss of Serine protease inhibitor Kazal-type 5 results in disrupted epidermal differentiation and increased inflammation, underscoring its protective role in barrier maintenance.\u003cbr\u003e\u003cbr\u003eBeyond dermatological function, Serine protease inhibitor Kazal-type 5 participates in immune regulation and mucosal defense. It modulates protease activity in airway and gastrointestinal epithelia, influencing inflammation and microbial interactions. The SPINK5 antibody supports analysis of these functions by allowing quantification of protein levels under inflammatory or allergic conditions. Studies suggest SPINK5 expression may also influence asthma and atopic dermatitis susceptibility, linking epithelial protease control to immune homeostasis.\u003cbr\u003e\u003cbr\u003eThe SPINK5 antibody performs effectively in western blotting, immunohistochemistry, and immunofluorescence, showing strong expression in the upper epidermis and secretory epithelia.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=SPINK5 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=SPINK5 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=SPINK5 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/SPINK5 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=SPINK5+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047286268269,"sku":"FY12394","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_68365114-d671-4ab2-8574-562856224c73.jpg?v=1782236990","url":"https:\/\/www.ebiohippo.com\/products\/spink5-antibody-serine-protease-inhibitor-kazal-type-5-bha17135297","provider":"BioHippo","version":"1.0","type":"link"}