{"product_id":"spock1-antibody-testican-1-bha17136175","title":"SPOCK1 Antibody \/ Testican 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eSPOCK1 Antibody \/ Testican 1 is a anti-SPOCK1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e SPOCK1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eSPOCK1 antibody detects Testican-1, a secreted glycoprotein that modulates extracellular matrix organization, protease activity, and cell migration. The UniProt recommended name is Testican-1 (SPOCK1). This protein belongs to the SPARC\/osteonectin family and contributes to tissue remodeling, neural development, and tumor invasion through regulation of extracellular proteolytic balance.\u003cbr\u003e\u003cbr\u003eFunctionally, SPOCK1 antibody identifies a 438-amino-acid protein characterized by domains for proteoglycan attachment and calcium binding. SPOCK1 inhibits metalloproteinases such as MMP2 and cathepsin L, thereby influencing extracellular matrix turnover. It also interacts with glycosaminoglycans and cell surface receptors to regulate adhesion, proliferation, and differentiation. In the nervous system, SPOCK1 supports axon guidance, synaptic stability, and neurogenesis. In non-neural tissues, it contributes to angiogenesis and wound repair.\u003cbr\u003e\u003cbr\u003eThe SPOCK1 gene is located on chromosome 5q31.2 and is expressed in brain, kidney, heart, and various epithelial tissues. Expression is regulated during embryogenesis and tissue regeneration and is modulated by growth factors including TGFbeta and EGF. SPOCK1's matrix-associated properties make it a key component in maintaining extracellular homeostasis.\u003cbr\u003e\u003cbr\u003ePathologically, elevated SPOCK1 expression has been linked to tumor progression and metastasis in cancers such as lung, liver, and prostate carcinoma. By modulating MMP activity and promoting epithelial-mesenchymal transition (EMT), SPOCK1 enhances invasion and angiogenesis. Conversely, in neural tissues, dysregulation affects axonal pathfinding and synapse formation. Research using SPOCK1 antibody supports studies in cancer metastasis, extracellular matrix biology, and neural development.\u003cbr\u003e\u003cbr\u003eSPOCK1 antibody can be validated for western blotting, immunohistochemistry, and ELISA to detect extracellular matrix proteins. \u003cbr\u003e\u003cbr\u003eStructurally, Testican-1 contains an N-terminal proteoglycan domain with glycosaminoglycan attachment sites, followed by Kazal-type protease inhibitor and calcium-binding domains. Its modular organization allows inhibition of proteolytic enzymes and stabilization of extracellular matrix components. This antibody enables analysis of SPOCK1's function in matrix remodeling, neural morphogenesis, and tumor biology.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=SPOCK1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=SPOCK1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=SPOCK1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/SPOCK1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=SPOCK1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047320248685,"sku":"FY13273","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_5a3db1e0-d361-4a0f-a0f5-87de5e750f9e.jpg?v=1782237078","url":"https:\/\/www.ebiohippo.com\/products\/spock1-antibody-testican-1-bha17136175","provider":"BioHippo","version":"1.0","type":"link"}