{"product_id":"spon1-antibody-spondin-1-bha17135410","title":"SPON1 Antibody \/ Spondin 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eSPON1 Antibody \/ Spondin 1 is a anti-SPON1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Immunohistochemistry (IHC), ELISA with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e SPON1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e IHC, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eSPON1 antibody detects Spondin-1, an extracellular matrix protein that promotes cell adhesion, axon guidance, and tissue organization. Spondin-1 is a member of the F-spondin family of secreted glycoproteins that regulate neuronal outgrowth and vascular patterning. The SPON1 antibody is used in neuroscience, developmental biology, and vascular research to study ECM signaling and neural development.\u003cbr\u003e\u003cbr\u003eSPON1 is encoded by the SPON1 gene located on human chromosome 11p15.2. The protein is approximately 91 kilodaltons and consists of a signal peptide, reelin domain, thrombospondin type I repeats, and a C-terminal region that mediates cell surface binding. Spondin-1 is secreted into the extracellular matrix where it interacts with integrins and heparan sulfate proteoglycans to promote neurite extension and cell attachment.\u003cbr\u003e\u003cbr\u003eA SPON1 antibody can detect a 90-95 kilodalton band by western blot and reveals extracellular and pericellular staining in immunohistochemistry. During embryonic development, Spondin-1 guides commissural axons across the midline and contributes to axonal fasciculation. In adult tissues, it participates in vascular remodeling and ECM integrity. Spondin-1 is also implicated in Alzheimer's disease pathology, where altered expression affects amyloid precursor protein processing.\u003cbr\u003e\u003cbr\u003eBeyond neural function, Spondin-1 contributes to chondrogenesis, osteogenesis, and tumor angiogenesis. Its expression is upregulated during wound repair and inflammation, suggesting a role in tissue regeneration.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=SPON1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=SPON1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=SPON1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/SPON1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=SPON1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047290593645,"sku":"FY12507","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_e7896e4b-118b-4263-a790-8915862aa7a2.jpg?v=1782237012","url":"https:\/\/www.ebiohippo.com\/products\/spon1-antibody-spondin-1-bha17135410","provider":"BioHippo","version":"1.0","type":"link"}